CyTOF 2 Maintenance

CyTOF 2 Maintenance15 minutes

Changing the Interface Pump Oil

  • Step 1

    Note: Perform this procedure before the instrument is turned on.

    Switch off the RF generator power using the RFG circuit breaker on the right side of the CyTOF® 2.

  • Step 2

    Open the front access door using the door handle. Pull the spring pins to open the instrument doors.

  • Step 3

    The Interface Pump is on the right side of the instrument. 

  • Step 4

    Verify the condition of the oil using the oil inspection chart. The oil should be below Level 4 as indicated.

    Open the valve on the Interface Pump and drain the oil into a tray or container.

    NOTE: If the oil is very dirty, fill 100 mL of fresh pump oil and then drain before proceeding to the next step.

  • Step 5

    Using a funnel, fill the oil.

    The oil level in the Interface Pump should be approximately ¾ full between the Min and Max lines on the visual inspection window.

  • Step 6

    Replace the cap and tighten with the Allen key. Do not overtighten.

  • Step 7

    Close the lower instrument door and the front access door of the CyTOF 2. Turn the vacuum switch back on.