245 results found for "primer"
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faq
Do you have any advice on primer design?
See these documents: …
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faq
How many primer pairs can I include in a multiplex preamplification reaction?
The Fluidigm preamplification protocol has been validated for multiplex preamplification of up to 96 targets. …
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faq
How should I store my Advanta NGS Library Prep Assay primers?
Primer pools are stable for up to one year at –20 °C if 96-well plates remain sealed to avoid evaporation. …
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faq
Can Fluidigm design assays targeting introns?
Yes. Please provide genomic coordinates in D3 assay design on the Fluidigm website. …
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faq
Will preamplification introduce amplification bias?
By limiting both the primer concentration and the number of PCR cycles, the preamplification protocol enables unbiased amplification of specific DNA targets. …
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faq
What is provided with my order of Advanta NGS Library Prep Assays?
Assays are provided in a 96-well plate, normalized to 2.5 μM per forward primer and 0.833 μM per reverse primer in each assay pool. The assay pools are provided in suspension buffer. Volume varies depending on assay order size. …
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faq
How many amplicons are generated by the Advanta CFTR NGS Library Prep Assay, and what is the size?
The Advanta™ CFTR NGS Library Prep Assay includes 73 assays organized into eight primer pools. …
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faq
What equipment is required for Access Array target-specific amplification?
The Access Array system is composed of two IFC Controller AX machines (one for pre-PCR lab and another for post-PCR lab) and an FC1 thermal cycler (PN AA-PKG-FC1). …
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product insert
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faq
What is the process for redesigning assays?
Redesigned primers or additional primers can be ordered through D3 at d3.fluidigm.com/account/login. …
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faq
Why is the Exo I treatment after preamplification needed only for EvaGreen?
EvaGreen® works by detecting all double-stranded DNA. …
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faq
How do I order Delta Gene assays?
Delta Gene assays can be ordered through D3 assay design from Fluidigm. …
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data sheet
Access Array Target Specific Primers FAQs
Access Array Target Specific Primers Data Sheet
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consumable
Access Array™ Target-Specific Primers
Custom amplicon tagging primers created using our D3™ assay design service. Sufficient primer volume for 100 48.48 Access Array IFCs. IFCs sold separately. …
Product #: ASY-AA
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faq
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faq
What is provided with my order of Access Array Target-Specific Primers?
Your order of Access Array Target-Specific Primers contains: …
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Quick Reference
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safety data sheet
2019-nCoV Probe & Primer RUO Kit
SDS-00026. Refers to part numbers 102-0687, 102-0688, 102-0689, 102-0690, 102-1324, 102-1325, 102-1326, 102-1327, 102-1328, 102-1329, 102-1330, 102-1331, 102-1332, 102-1333, 102-1334, 102-1335, 102-1336, 102-1337. Applies to components in part numbers 102-0690, 102-1340, 102-1341.
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faq
What are Access Array Target-Specific Primers?
Access Array Target-Specific Primers are designed by the Fluidigm Assay Design Group for targeted amplicon sequencing using next-generation sequencing platforms. …
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consumable
Access Array™ Multiplex Target-Specific Primers
Custom multiplexed amplicon tagging primers created using our D3™ assay design service. Sufficient primer volume for 100 48.48 Access Array IFCs. IFCs sold separately. …
Product #: ASY-AAX