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Gene Expression and Genotyping IFC Family Guide

With the Biomark™ HD System and its family of IFCs (integrated fluidic circuits), you get high sample throughput, ultra-high data quality, assay flexibility, and an easy workflow—everything you need in one solution.

faq

What is the shelf life for IFCs and Control Line Fluid?

In March 2019, Fluidigm began adding use-by dates (expiration dates) to the labeling of product packaging for new batches of IFCs and Control Line Fluid. The assigned use-by date for each product is based on the results of real-time stability studies and is relative to the date of batch manufacture. Most products are stable for two years from the data of manufacture. …

faq

What is the difference between the Genotyping and Gene Expression IFCs?

Gene Expression and Genotyping IFCs are offered in the same formats (e.g., 48.48, 96.96, 192.24 and Flex Six) but do not necessarily have the same reaction chamber volumes per application type. …

faq

Can the IFCs be centrifuged to get rid of bubbles?

Do not centrifuge an IFC, as centrifugation might damage the IFC. To avoid introducing bubbles: …

faq

Can I reuse an IFC? Can I use part of an IFC, and then use the other part in another experiment?

All IFCs except the Flex Six IFC are single-use only, and the entire IFC must be filled with reagents when used. Flex Six IFCs (available in gene expression and genotyping formats) contain six single-use partitions within the same IFC. …

faq

How quickly do I need to use the IFC after the foil pouch is opened?

IFCs must be used within 24 hours of opening the sealed foil pouch. …

faq

How should I store Flex Six IFCs between use?

Between uses, Flex Six IFCs should be stored as follows: …

faq

Can I use the LP 192.24 IFC on the AX Controller?

No, the LP 192.24 IFC was designed to work specifically with the Advanta™ NGS Library Prep Reagent Kit on Juno™ and is not compatible with the AX Controller. …

faq

What are the software and firmware requirements for Flex Six IFC?

For the Flex Six Gene Expression IFC: …

faq

How much time can elapse between loading an IFC and thermal cycling?

Because IFCs are made of a gas-permeable material, it is best to start PCR on the Biomark HD system or the FC1 cycler immediately to avoid IFC dehydration or reagent evaporation. …

faq

Can the 24.192 GE IFC be used for genotyping?

Though Fluidigm does not make a 24.192 IFC for genotyping, Fluidigm scientists have shown that the 24.192 GE IFC can be used for genotyping using SNP Type chemistry. …