691 results found for "IFC"
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faq
What is the shelf life for IFCs and Control Line Fluid?
In March 2019, Fluidigm began adding use-by dates (expiration dates) to the labeling of product packaging for new batches of IFCs and Control Line Fluid. The assigned use-by date for each product is based on the results of real-time stability studies and is relative to the date of batch manufacture. Most products are stable for two years from the data of manufacture. …
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faq
What is the difference between the Genotyping and Gene Expression IFCs?
Gene Expression and Genotyping IFCs are offered in the same formats (e.g., 48.48, 96.96, 192.24 and Flex Six) but do not necessarily have the same reaction chamber volumes per application type. …
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faq
Can I reuse an IFC? Can I use part of an IFC, and then use the other part in another experiment?
All IFCs except the Flex Six IFC are single-use only, and the entire IFC must be filled with reagents when used. Flex Six IFCs (available in gene expression and genotyping formats) contain six single-use partitions within the same IFC. …
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faq
Can the IFCs be centrifuged to get rid of bubbles?
Do not centrifuge an IFC, as centrifugation might damage the IFC. To avoid introducing bubbles: …
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faq
How quickly do I need to use the IFC after the foil pouch is opened?
IFCs must be used within 24 hours of opening the sealed foil pouch. …
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How should I store Flex Six IFCs between use?
Between uses, Flex Six IFCs should be stored as follows: …
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faq
Can I use the LP 192.24 IFC on the AX Controller?
No, the LP 192.24 IFC was designed to work specifically with the Advanta™ NGS Library Prep Reagent Kit on Juno™ and is not compatible with the AX Controller. …
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faq
What are the software and firmware requirements for Flex Six IFC?
For the Flex Six Gene Expression IFC: …
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faq
How much time can elapse between loading an IFC and thermal cycling?
Because IFCs are made of a gas-permeable material, it is best to start PCR on the Biomark HD system or the FC1 cycler immediately to avoid IFC dehydration or reagent evaporation. …
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faq
Can the 24.192 GE IFC be used for genotyping?
Though Fluidigm does not make a 24.192 IFC for genotyping, Fluidigm scientists have shown that the 24.192 GE IFC can be used for genotyping using SNP Type chemistry. …
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faq
If I have multiple IFC runs, can I prepare my sample mix and assay mix the day before? A week before?
Sample mixes should be prepared the same day the IFC is loaded. Once prepared, never freeze and thaw sample mixes. …
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faq
How do Ct values on the 24.192 GE IFC differ from values obtained on the Biomark 96.96 GE IFC?
Because of the difference in chamber size between the 2 IFCs, you can expect to see a shift in threshold cycle (Ct) values. …
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faq
Can I purchase the 24.192 GE IFC independently from the reagent kit?
Yes. The IFC can be purchased together with the control line fluid in packs of 2 (PN 101-7995) and 10 (PN 101-7996). …
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faq
Can I use my existing Access Array kit and assays on Juno with the Juno LP 192.24 IFC?
No, the LP 192.24 IFC was designed to work specifically with the Advanta™ NGS Library Prep Reagent Kit on Juno™. …
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faq
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consumable
LP 48.48 IFC—5 IFCs
Five Juno LP 48.48 IFCs for library preparation. Control Line Fluid and reagents sold separately. …
Product #: 101-2343
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consumable
LP 48.48 IFC—10 IFCs
Ten Juno LP 48.48 IFCs for library preparation. Control Line Fluid and reagents sold separately. …
Product #: 101-2342
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faq
What is the difference between the Biomark 24.192 Complete Bundle and the 24.192 GE IFC & Reagent Kit?
The 24.192 GE IFC & Reagent Kit does not contain the Preamp Master Mix, the Reverse Transcription Master Mix, and PCR Water. …
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faq
Is the 24.192 GE IFC compatible with Fast TaqMan chemistry?
The 24.192 GE IFC can be used with Fast TaqMan® chemistry. …