899 results found in Single-Cell Proteomics
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system
Helios, a CyTOF System
The highest-resolution profiling of cell phenotype and function available …
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faq
Why is the use of CAS and WB Injector only recommended for Helios? Wouldn’t samples run on any mass cytometer benefit from being resuspended in CAS?
Although we have not rigorously tested the use of Cell Acquisition Solution (CAS) with samples run on CyTOF® 2 systems, it is likely that some benefit to cell signal strength and stability would be seen on those systems as well. …
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faq
How is this new cell staining protocol different from previous Fluidigm protocols, and what makes it better?
The biggest change is that the updated protocols all call for use of freshly prepared 1.6% formaldehyde solution. Fluidigm recommends using ampules of Pierce™ 16% (w/v) Formaldehyde, Methanol-free (Thermo Scientific™ Cat. No. 28906, 10 x 1 mL). Other sources of fixative are available. Please contact your field applications scientist for more information. Optimal staining and cell integrity is only obtained using fixative prepared from a new ampule daily. …
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faq
Is the cleaning procedure any different for this new injector?
The procedure is the same. However, the WB Injector needs more frequent cleaning than the HT Injector. …
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faq
What are the new protocols for mass cytometry that I have been hearing about?
Fluidigm has released updated protocols for all cell staining and fixation procedures. The most important update is the use of freshly prepared formaldehyde fixative each day that cells are stained. The updated protocols can be found here. …
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faq
How do I order the new injector? How much does it cost?
The first WB Injector is supplied free of charge along with a supply of Maxpar® Cell Acquisition Solution. Please contact your field application scientist (FAS) if you would like to try out the new Helios™ sample acquisition protocol and your FAS will help you place the order. Additional injectors can be purchased through Fluidigm by referencing Cat. No. 107950. …
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User Guide
Helios User Guide
This guide is intended for users and operators of the Helios™ instrument. The guide provides an introduction to mass cytometry and the Helios system.
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Protocol
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Protocol
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safety data sheet
Metal-Conjugated Antibodies
3000000X-3999999X - Metal-Conjugated Antibodies
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CyTOF Software
CyTOF® 6.7 system control software is designed to be user-friendly and intuitive for system control, and to provide continuous instrument feedback and diagnostic capabilities to users. This gives users the ability to troubleshoot quickly and effectively as well as to provide the highest quality of data output. …
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tds
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tds
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article
Unlocking Topographic Biomarkers
The interactive histoCAT toolbox unifies the cytometry and imaging communities …
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Publications
Mass Cytometry Bibliography
Publications featuring mass cytometry technology
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article
600 Points of Insight for Cellular Phenotyping
Expand your mass cytometry universe with hundreds of new Maxpar antibodies …
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specification
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specification
CyTOF 2 Mass Cytometry Specification Sheet
This document provides minimum specifications and an overview of site requirements for the CyTOF 2 System.
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Poster
IMMUNOLOGY 2014: Analysis of Mass Cytometry Data with viSNE Reveals Phenotypic Heterogeneity with Human Tregs
viSNE analysis of human Tregs clearly identifies naïve, effector, terminal effector, and Helios- populations of Tregs and will be of significant use for further deep phenotyping of human Treg subsets.
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Software