962 results found in Single-Cell Proteomics
-
resource
Fluidigm Cytobank Analysis
Cytobank cloud-based data analysis software turns high-content data into high-impact knowledge by providing tools optimized for mass cytometry, including clustering (SPADE and viSNE), heat maps, dot plots, histograms and statistics, along with the ability to share your data with colleagues and collaborators. …
-
User Guide
Helios User Guide
This guide is intended for users and operators of the Helios™ instrument. The guide provides an introduction to mass cytometry and the Helios system.
-
faq
How do I install the new injector?
A customer bulletin, New Sample Acquisition Protocols (PN 400275), on installation, use, and care of the WB Injector is available online. …
-
faq
What are the new protocols for mass cytometry that I have been hearing about?
Fluidigm has released updated protocols for all cell staining and fixation procedures. The most important update is the use of freshly prepared formaldehyde fixative each day that cells are stained. The updated protocols can be found here. …
-
faq
Why do I have to use the new Maxpar Cell Acquisition Solution? Why can’t I just keep using water?
You do not have to use the Maxpar® Cell Acquisition Solution (CAS; Cat. No. 201237). However, its development was driven by a desire to create a more favorable environment for cells than provided by resuspension in Maxpar Water. We are committed to continually improving quality, and therefore we carefully devised a new running solution. …
-
faq
I heard that a new injector and running solution can be used on our mass cytometer. Should I ask my operator to use those when I run my next experiment?
All facilities with Helios™ and CyTOF® 2-to-Helios Upgrade mass cytometers are being informed of a new sample acquisition protocol that has been shown in some cases to improve intensities and CVs on signals from metal isotope-stained cells. The protocol uses a higher-ionic-strength running solution, called Maxpar® Cell Acquisition Solution (CAS; Cat. No. 201237). …
-
faq
Can this new sample acquisition protocol be used on any mass cytometer?
The use of the WB Injector and Cell Acquisition Solution has only been validated on Helios™ and CyTOF® 2-to-Helios Upgrade mass cytometers. …
-
faq
Why is the use of CAS and WB Injector only recommended for Helios? Wouldn’t samples run on any mass cytometer benefit from being resuspended in CAS?
Although we have not rigorously tested the use of Cell Acquisition Solution (CAS) with samples run on CyTOF® 2 systems, it is likely that some benefit to cell signal strength and stability would be seen on those systems as well. Internal testing is underway on CyTOF 2 systems. …
-
faq
Is the cleaning procedure any different for this new injector?
The procedure is the same. However, the WB Injector needs more frequent cleaning than the HT Injector. …
-
faq
How can I tell the difference between the new injector and my existing injector?
The new WB Injector is inscribed with the letters WB near the base of the injector in front of the ball joint. …
-
faq
Should I implement the updated cell staining protocols immediately?
If you are starting any new studies or sets of experiments, we recommend that you use the updated staining protocols. If new experiments are to be part of an ongoing study, we strongly suggest that you perform a bridging study first. Contact your Fluidigm field applications scientist if you are unsure how to proceed with such studies. …
-
faq
How is this new cell staining protocol different from previous Fluidigm protocols, and what makes it better?
The biggest change is that the updated protocols all call for use of freshly prepared 1.6% formaldehyde solution. Fluidigm recommends using ampules of Pierce™ 16% (w/v) Formaldehyde, Methanol-free (Thermo Scientific™ Cat. No. 28906, 10 x 1 mL). Other sources of fixative are available. Please contact your field applications scientist for more information. Optimal staining and cell integrity is only obtained using fixative prepared from a new ampule daily. …
-
faq
How do I order the new injector? How much does it cost?
The first WB Injector is supplied free of charge along with a supply of Maxpar® Cell Acquisition Solution. Please contact your field application scientist (FAS) if you would like to try out the new Helios™ sample acquisition protocol and your FAS will help you place the order. Additional injectors can be purchased through Fluidigm by referencing Cat. No. 107950. …
-
tds
-
tds
-
tds
-
Publications
Bayesian Hierarchical Models for Protein Networks in Single-Cell Mass Cytometry
We propose a class of hierarchical models to investigate the protein functional network of cellular markers. …
-
Protocol
-
tds
-
brochure
Cell-ID 20-Plex Pd Barcoding Kit Brochure
Ask bigger questions. Get higher-confidence answers.