Efficiently scale your targeted next-generation sequencing (NGS) library preparation with the Juno™ system and the Targeted DNA Sequencing Library Prep reagents.
Achieve scalable throughput from tens to hundreds of NGS-ready libraries per processing run with two IFC formats – LP 48.48 IFC and Juno LP 192.24 IFC.
Reduce hands-on time and drive down reagent consumption with automated microfluidics, resulting in significant savings. And to ensure that your NGS panel always remains up-to-date, maintain flexible customization capabilities unique to Fluidigm.
What's New
Spotlight
DATA SHEET | Juno Targeted DNA Sequencing Library Preparation System
Customized assay panels in convenient kits provide investigators and genomics service providers with the industry’s only easily scalable and automated solution. Targeted DNA Sequencing Library Preparation is compatible with Illumina® MiniSeq™, MiSeq™, NextSeq™ and HiSeq® NGS systems.
What's New
FEATURE | Advanta CFTR NGS Library Prep Assay saves time and money through an efficient and scalable targeted NGS workflow
The Advanta™ CFTR NGS Library Prep Assay automates targeted enrichment of cystic fibrosis transmembrane conductance regulator (CFTR) variants from each of the gene’s 27 exons and select intronic regions using proven microfluidics technology. The workflow significantly lowers hands-on time while conserving precious reagents by reducing reactions to the nanoliter scale. With the capacity to produce from 48 to over 1,500 NGS libraries per week, scalability is simplified to meet laboratory needs as they change over time. Protocols are available that support Juno™ and Access Array™ systems.
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Advanta CFTR NGS Library Prep Assay Flyer |
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Advanta Evidence-Based CFTR Variant Coverage Flyer |
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Methods
Juno Targeted DNA Sequencing Library Preparation
Scale with the industry’s most flexible per-sample throughput and enrichment capabilities using the Juno targeted DNA Sequencing Library Preparation System. Two sample throughput options are available, LP 48.48 and Juno LP 192.24 IFC (integrated fluidic circuit).
The LP 192.24 IFC automates amplicon based libraries for 192 unique DNA samples per run and up to 2,400 amplicon targets per sample. The LP 48.48 IFC accommodates up to 48 DNA samples per run and up to 4,800 amplicon targets per sample. Targeted DNA Sequencing Library Preparation is fully compatible with all Illumina® sequencing systems. Up to 1,536 sample libraries may be multiplexed per sequencing run.
Access Array Library Preparation
Access Array™ delivers specimen flexibility supporting sample types of DNA extracted from germline and FFPE somatic samples. Access Array automates targeted DNA library preparation for 48 unique DNA samples per run and up to 4,800 amplicon targets per sample using Targeted DNA Seq Library reagents, or up to 480 amplicon targets per sample using Access Array chemistry.
The system is compatible with multiple sequencing platforms including Illumina® sequencing systems and, for use with Access Array chemistry, Ion Torrent™.
Products
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Reagents
Empower your lab with reagents designed to provide fast, precise results.
- Access Array
Product # Product name 100-1032 48.48 Access Array™ Loading Reagent Kit with Control Line Fluid—10 IFCs DescriptionSufficient Control Line Fluid syringes and reagents to prepare 48 unique amplicons over ten 48.48 Access Array IFCs. IFCs sold separately.
100-1032-R 48.48 Access Array™ Loading Reagent Kit—10 IFCs DescriptionSufficient reagents to prepare 48 unique amplicons over ten 48.48 Access Array IFCs. IFCs and Control Line Fluid syringes sold separately.
100-3155 Access Array™ Harvest Solution—5 Bottles DescriptionSufficient harvest solution for ten 48.48 Access Array IFCs. IFCs and Control Line Fluid syringes sold separately.
100-6223 48.48 Access Array™ Hydration Reagent—10 mL DescriptionHydration Reagent for 48.48 Access Array IFCs. IFCs sold separately.
100-7604 20X Access Array™ Loading Reagent—2 Tubes DescriptionSufficient loading reagent for twenty 48.48 Access Array IFCs. IFCs sold separately.
100-7966 Access Array™ Hydration Reagent v2—5 mL DescriptionReagent for use with Access Array IFCs. IFCs sold separately. Not a sellable part?
101-0915 Bulk 20X Access Array™ Loading Reagent—25 mL DescriptionBulk reagent for use with Access Array IFCs. IFCs sold separately.
101-0740 Targeted DNA Seq Assay Loading Reagent—5 Tubes DescriptionSufficient reagent for 10 Juno LP 192.24 IFCs. IFCs and Control Line Fluid syringes sold separately.
101-0741 Targeted DNA Seq Harvest Reagent—5 Bottles DescriptionSufficient reagent for 10 Juno™ LP 192.24 IFCs. IFCs and Control Line Fluid syringes sold separately.
101-0742 Targeted DNA Seq Adapter Mix—5 Tubes DescriptionSufficient reagent for 10 Juno™ LP 192.24 IFCs. IFCs and Control Line Fluid syringes sold separately.
100-4876 Access Array™ Barcode Library for Illumina® Sequencers—384, Single Direction Description384 barcodes for single-direction single-read or paired-end sequencing on Illumina® sequencers. Sufficient volume to run ten 384 barcoding reactions.
101-0744 Targeted DNA Seq Barcode Plates DescriptionNecessary barcodes to support 40 Juno™ LP 192.24 IFCs. IFCs, Control Line Fluid syringes and reagents sold separately.
101-2412 Targeted DNA Seq Library Adapter Set DescriptionUnique adapters to enable dual indexing, resulting in up to 1536 combinations. Sufficient for 40 LP—192.24 IFCs or 40 LP—48.48 IFCs. IFCs and Control Line Fluid syringes sold separately.
101-7779 Advanta™ NGS Library Prep Core Kit—LP 48.48, 10 IFCs DescriptionThis kit provides the necessary IFCs, reagents, and consumables to support next-generation sequencing (NGS) library preparation on the LP 48.48 IFC. The kit contains sufficient materials for 10 LP 48.48 IFCs.
- Juno
Product # Product name 101-0740 Targeted DNA Seq Assay Loading Reagent—5 Tubes DescriptionSufficient reagent for 10 Juno LP 192.24 IFCs. IFCs and Control Line Fluid syringes sold separately.
101-0741 Targeted DNA Seq Harvest Reagent—5 Bottles DescriptionSufficient reagent for 10 Juno™ LP 192.24 IFCs. IFCs and Control Line Fluid syringes sold separately.
101-0742 Targeted DNA Seq Adapter Mix—5 Tubes DescriptionSufficient reagent for 10 Juno™ LP 192.24 IFCs. IFCs and Control Line Fluid syringes sold separately.
101-0744 Targeted DNA Seq Barcode Plates DescriptionNecessary barcodes to support 40 Juno™ LP 192.24 IFCs. IFCs, Control Line Fluid syringes and reagents sold separately.
101-2412 Targeted DNA Seq Library Adapter Set DescriptionUnique adapters to enable dual indexing, resulting in up to 1536 combinations. Sufficient for 40 LP—192.24 IFCs or 40 LP—48.48 IFCs. IFCs and Control Line Fluid syringes sold separately.
101-7779 Advanta™ NGS Library Prep Core Kit—LP 48.48, 10 IFCs DescriptionThis kit provides the necessary IFCs, reagents, and consumables to support next-generation sequencing (NGS) library preparation on the LP 48.48 IFC. The kit contains sufficient materials for 10 LP 48.48 IFCs.
101-7782 Advanta™ NGS Library Prep Core Kit—LP 192.24, 2 IFCs DescriptionThis kit provides the necessary IFCs, reagents, and consumables to support next-generation sequencing (NGS) library preparation on the LP 192.24 IFC. The kit contains sufficient materials for two LP 192.24 IFCs.
101-7783 Advanta™ NGS Library Prep Core Kit—LP 192.24, 10 IFCs DescriptionThis kit provides the necessary IFCs, reagents, and consumables to support next-generation sequencing (NGS) library preparation on the LP 192.24 IFC. The kit contains sufficient materials for 10 LP 192.24 IFCs.
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Standard Panels
- Access Array
Product # Product name 100-5451 BRCA1/BRCA2/TP53 Target-Specific Panel DescriptionAccess Array™ targeted sequencing primers for all exons in the human BRCA1, BRCA2 and TP53 genes.
100-5495 BRCA1 Target-Specific Panel DescriptionAccess Array™ targeted sequencing primers for all exons in the human BRCA1 gene.
100-5496 BRCA2 Target-Specific Panel DescriptionAccess Array™ targeted sequencing primers for all exons in the human BRCA2 gene.
100-5497 EGFR Target-Specific Panel DescriptionAccess Array™ targeted sequencing primers for all exons in the human EGFR gene.
100-5498 MET Target-Specific Panel DescriptionAccess Array™ targeted sequencing primers for all exons in the human MET gene.
100-5499 TP53 Target-Specific Panel DescriptionAccess Array™ targeted sequencing primers for all exons in the human TP53 gene.
100-6060 EGFR/MET Target-Specific Panel DescriptionAccess Array™ targeted sequencing primers for all exons in the human EGFR and MET genes.
- Juno
Product # Product name 101-7033 Advanta™ Solid Tumor NGS Library Prep Assay DescriptionThis kit enables the preparation of NGS-ready libraries utilizing DNA isolated from FFPE solid tumor samples for subsequent sequencing analysis. The kit includes sufficient targeted DNA sequencing library reagents, Solid Tumor Assay Pools, LP 8.8.6 IFCs, and Control Line Fluid to generate up to 96 sample libraries.
101-8654 Advanta™ RNA Fusions NGS Library Prep Assay DescriptionThis kit enables the preparation of NGS-ready libraries utilizing RNA isolated from FFPE solid tumor samples for subsequent sequencing analysis. The kit includes sufficient targeted RNA sequencing library reagents, RNA Fusions Assay Pools, LP 8.8.6 IFCs, and Control Line Fluid to generate up to 96 sample libraries.
101-6156 Advanta™ CFTR NGS Library Prep Assay—LP 48.48, 2 IFCs DescriptionThis kit enables the preparation of NGS-ready libraries from 27 exons and select intronic regions of the cystic fibrosis transmembrane conductance regulator gene (CFTR) for subsequent sequencing analysis. The kit includes sufficient targeted DNA sequencing library reagents, CFTR NGS Assay Pools, IFCs, and Control Line Fluid to generate 96 libraries. Product insert available.
101-7727 Advanta™ CFTR NGS Library Prep Assay with Preamp—LP 48.48, 2 IFCs DescriptionThis kit enables the preparation of NGS-ready libraries from 27 exons and select intronic regions of the cystic fibrosis transmembrane conductance regulator (CFTR) gene for subsequent sequencing analysis. The kit includes sufficient targeted DNA sequencing library reagents, CFTR NGS Assay Pools, IFCs, and Control Line Fluid to generate 96 libraries. The kit also includes sufficient reagents and primers for target enrichment of DNA from samples with lower yield or DNA quality. Product insert available.
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Control Line Fluid
Product # Product name 89000020 Control Line Fluid Kit—48.48 DescriptionSufficient Control Line Fluid syringes for ten 48.48 Access Array™ IFCs or 48.48 Dynamic Array™ IFCs for both genotyping and gene expression. IFCs and reagents sold separately.
101-2345 LP Control Line Fluid 48.48—5 Pack DescriptionSufficient Control Line Fluid syringes for five Juno LP 48.48 IFCs. IFCs and reagents sold separately.
101-2344 LP Control Line Fluid 48.48—10 Pack DescriptionSufficient Control Line Fluid syringes for ten Juno LP 48.48 IFCs. IFCs, reagents and barrier tape applicator sold separately.
101-2016 Juno™ Control Line Fluid 192.24—5 Pack DescriptionSufficient Control Line Fluid syringes for 5 Juno LP 192.24 IFCs. IFCs and reagents sold separately.
100-7131 Juno™ Control Line Fluid 192.24—10 Pack DescriptionSufficient Control Line Fluid syringes for 10 Juno LP 192.24 IFCs. IFCs and reagents sold separately.
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IFCs
Combine your experimental protocols into a single workflow with advanced microfluidics technology IFCs.
Product # Product name 101-1926 LP 48.48 IFC DescriptionOne Juno LP 48.48 IFC for library preparation. Control Line Fluid and reagents sold separately.
101-2343 LP 48.48 IFC—5 IFCs DescriptionFive Juno LP 48.48 IFCs for library preparation. Control Line Fluid and reagents sold separately.
101-2342 LP 48.48 IFC—10 IFCs DescriptionTen Juno LP 48.48 IFCs for library preparation. Control Line Fluid and reagents sold separately.
- Access Array
Product # Product name 101-2349 LP 48.48 IFC with Accessories—5 Pack DescriptionFive Juno LP 48.48 IFCs and sufficient Control Line Fluid syringes and barrier tapes. Reagents and barrier tape applicator sold separately.
101-2348 LP 48.48 IFC with Accessories—10 Pack DescriptionTen Juno LP 48.48 IFCs and sufficient Control Line Fluid syringes and barrier tapes. Reagents and barrier tape applicator sold separately.
- Juno
Product # Product name 101-1824 Juno™ LP 192.24 IFC DescriptionOne Juno LP 192.24 IFC for library preparation. Control Line Fluid and reagents sold separately.
101-2014 Juno™ LP 192.24 IFC—5 IFCs DescriptionFive Juno LP 192.24 IFCs for library preparation. Control Line Fluid and reagents sold separately.
101-1826 Juno™ LP 192.24 IFC—10 IFCs DescriptionTen Juno LP 192.24 IFCs for library preparation. Control Line Fluid and reagents sold separately.
101-2334 Juno™ LP 192.24 IFC with Accessories—5 Pack DescriptionFive Juno LP 192.24 IFCs and sufficient Control Line Fluid syringes and barrier tape. Reagents and barrier tape applicator sold separately.
101-2333 Juno™ LP 192.24 IFC with Accessories—10 Pack DescriptionTen Juno LP 192.24 IFCs and sufficient Control Line Fluid syringes and barrier tapes. Reagents and barrier tape applicator sold separately.
101-2349 LP 48.48 IFC with Accessories—5 Pack DescriptionFive Juno LP 48.48 IFCs and sufficient Control Line Fluid syringes and barrier tapes. Reagents and barrier tape applicator sold separately.
101-2348 LP 48.48 IFC with Accessories—10 Pack DescriptionTen Juno LP 48.48 IFCs and sufficient Control Line Fluid syringes and barrier tapes. Reagents and barrier tape applicator sold separately.
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Accessories
- Access Array
Product # Product name 100-5920 Access Array™ Accessory Kit DescriptionSufficient barrier tapes for twenty 48.48 Access Array IFCs and tool for applying and removing tape. IFCs sold separately.
- Juno
Product # Product name 101-9226 LP 8.8.6 IFC with Accessories - 2 Pack DescriptionTwo Juno LP 8.8.6 IFCs and sufficient control line fluid and barrier tapes.
101-2017 Juno™ LP 192.24 Barrier Tape—5 Pack DescriptionSufficient barrier tapes for 5 Juno LP 192.24 IFCs. IFCs and barrier tape applicator sold separately.
101-1825 Juno™ LP 192.24 Barrier Tape—10 Pack DescriptionSufficient barrier tapes for 10 Juno LP 192.24 IFCs. IFCs and barrier tape applicator sold separately.
101-2027 Juno™ LP Barrier Tape Applicator DescriptionBarrier tape applicator for Juno LP IFCs. IFCs and barrier tapes sold separately.
101-2347 Juno™ LP 48.48 Barrier Tape—5 Pack DescriptionSufficient barrier tapes for five Juno LP 48.48 IFCs. IFCs and barrier tape applicator sold separately.
101-2346 Juno™ LP 48.48 Barrier Tape—10 Pack DescriptionSufficient barrier tapes for ten Juno LP 48.48 IFCs. IFCs and barrier tape applicator sold separately.
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Assay Design
Simplify your customized targeted DNA library prep assay design with the D3™ primer design portal services provided by our Fluidigm Assay Design Group (ADG). Get started online anytime, or contact adg@fluidigm.com to team with our in-house experts for complimentary one-on-one assay design support.
- Juno
Product # Product name ASY-MPX Targeted DNA Seq Library Assays Design DescriptionCustom-designed assay for targeted NGS using Access Array™ or Juno™ system. Assay provided in singleplex format, 450 μM/150 μM forward and reverse. Reagent kits and IFCs sold separately. Turnaround time 3–4 weeks.
ASY-MPX-P Targeted DNA Seq Library Assays (Pooled) Design DescriptionCustom-designed assay for targeted NGS using Access Array™ or Juno™ system. Assays provided in singleplex format, 450 μM/150 μM forward and reverse. One plate of pre-pooled primers of entire order (Fluidigm job ID required) is included (2.5 μM/0.833 μM). Reagent kits and IFCs sold separately. Turnaround time 5–6 weeks. Minimum 240 assays per order.