Akt, also known as PKB or Rac, is an intracellular serine/threonine protein kinase critically involved in several pathways including apoptosis, cell cycle, and energy regulation. Akt integrates growth factor signaling through PI3K and is activated in a two-step process involving a priming phosphorylation at Thr308 by PDK1 followed by phosphorylation at Ser473 by mTOR complex 2 (mTORC2). Activated Akt promotes protein translation and cell growth by activating mTOR complex 1 (mTORC1). Akt prevents degradation of cyclin D1, thereby promoting G1 entry. Akt also promotes cell survival by inactivating the pro-apoptotic protein BAD.
Anti-pAkt [S473] (D9E)-152Sm—50 Tests