Mass cytometry, powered by CyTOF® technology , is the world’s most advanced single-cell proteomics technology. It has opened new doors to discovery by empowering researchers to interrogate more than 50 markers simultaneously on millions of individual cells, to unveil new cell types, functions and biomarkers in immunology, cancer, stem cells and more.
Fluidigm Maxpar® reagents and protocols along with our Maxpar Panel Designer tool provide all you need for groundbreaking and cost-effective experiments that reveal systems-level biology at single-cell resolution.

The Helios mass cytometer uses the well-established technology of inductively coupled plasma mass spectrometry while handling sample preparation, sample introduction and data output in a manner similar to flow cytometry.
What's New

Flow Cytometer Trade-In Program
For a limited time, trade in any flow cytometer for up to a 30% discount on the purchase of a Helios™.
Switch to single-tube cytometry to accelerate your research with the most comprehensive view of cell phenotype and function.
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Cadmium-labeled CD45 Antibodies for Live Cell Barcoding |
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Flexible Panel Design with Cadmium Metal Tags |
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Methods
Sample Types
Current CyTOF systems, including the latest mass cytometer, Helios™, provide high-parametric data on individual cells prepared in liquid suspension. Standard protocols for preparing single-cell suspensions from tissue culture and solid and liquid tissues as well as tumors can be used for CyTOF sample prep. Sample size can vary from thousands to millions of cells.
Metal-Tagged Reagents
Cellular targets in liquid suspension can be labeled with more than 50 metal-tagged reagents, including antibodies, nucleic acid intercalators and analogs, as well as other biochemical ligands. Each reagent is detected and quantified with cytometry by time-of-flight mass spectrometry in the CyTOF system. The high purity and choice of metal isotopes ensure minimal background noise from signal overlap or endogenous cellular components. Our catalog is stocked with over 800 antibodies detecting more than 400 unique human or mouse targets. If a desired antibody is not yet present in our catalog, you can make it yourself using our metal-labeling kits or custom order biologically validated material using our custom conjugation services.
Cell Profiling
Targets can be anywhere in the cell, including the surface, cytoplasm, nucleus or internal organelles. Complete profiling allows CyTOF researchers to investigate a range of applications, including
- Phenotyping with CD and cell-surface antibodies
- Signaling and transcription factors
- Cytokines
- Cell death and apoptosis
- Cell cycle and proliferation
- Structure and cytoskeleton
- Enzymatic activity
Phosphoprotein detection can be used to map the signaling profile of samples, and optimized protocols and detailed technical assistance are available for any combination of targets.
Sample Barcoding
Sample barcoding is a powerful tool offering increased throughput, improved protocol consistency and enhanced data quality. Barcoding also enables efficient use of small precious samples through multiplexing and may provide cost savings through reduced consumption of reagents. Two approaches are available for CyTOF:
- Cell-ID™ 20-Plex Pd Barcoding Kit for fixed cell samples
- Cd-CD45 antibodies for live cell samples
Products
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Helios™, a CyTOF® system
Deeply profile cell phenotypes and functional states using CyTOF technology, all in a single tube. .
Helios Explore -
Maxpar Metal-Conjugated Antibodies
The Maxpar catalog features more than 800 metal-labeled antibodies and continues to grow. The broad selection significantly expands possibilities for discovery and monitoring on Fluidigm mass cytometry systems. Design your own custom panel to analyze immunity, cancer progression, cancer immunotherapy or other disease research targets.
Primary Antibodies Explore fluidigm Secondary Antibodies Explore -
Cell-ID
Mass cytometry provides researchers with the power to simultaneously interrogate dozens of parameters on millions of cells. The Cell-ID™ 20-Plex Pd Barcoding Kit vastly expands your throughput for more robust data revealing subtle phenotypes.
- Barcodes
Product # Product name 201060 Cell-ID™ 20-Plex Pd Barcoding Kit DescriptionThe kit enables unique barcoding of 20 samples so they can be combined and subsequently stained and acquired as one multiplexed sample, followed by software debarcoding and individual sample analysis.
- Cell-ID
Product # Product name 201064 Cell-ID™ Cisplatin—100 µL DescriptionCell-ID Cisplatin specifically identifies dead cells when incubated prior to fixation or identifies total cells when incubated following cell fixation and permeabilization. Containing a mixture of naturally occurring platinum isotopes, it is best detected in the 195 channel.
201194 Cell-ID™ Cisplatin-194Pt—100 µL DescriptionCell-ID™ Cisplatin-194Pt is a monoisotopic preparation of cisplatin-containing the 194Pt isotope. Cisplatin is a stain that binds covalently to cellular proteins and labels cells with compromised cell membranes to a greater extent than live cells. Cell-ID Cisplatin-194Pt therefore specifically identifies dead cells if cells are incubated prior to fixation, or it identifies total cells if cells are incubated following cell fixation and permeabilization. Because cisplatin binds covalently to protein, its labeling remains strong through subsequent cell handling steps used in downstream mass cytometry cell staining protocols.
201198 Cell-ID™ Cisplatin-198Pt—100 µL DescriptionCell-ID™ Cisplatin-198Pt is a monoisotopic preparation of cisplatin-containing the 198Pt isotope. Cisplatin is a stain that binds covalently to cellular proteins, and labels cells with compromised cell membranes to a greater extent than live cells. Cell-ID Cisplatin-198Pt therefore specifically identifies dead cells if cells are incubated prior to fixation, or it identifies total cells if cells are incubated following cell fixation and permeabilization. Because cisplatin binds covalently to protein, its labeling remains strong through subsequent cell handling steps used in downstream mass cytometry cell staining protocols.
201127 Cell-ID™ IdU, 100 µL—300 Tests DescriptionCell-ID 127 IdU incorporates into DNA of proliferating cells and is a marker of S phase of the cell cycle.
201103A Cell-ID™ Intercalator-Rh—500 µM DescriptionA cationic nucleic acid intercalator that is used in CyTOF® analysis to discriminate dead cells from live cells or single nucleated cells from doublets.
201103B Cell-ID™ Intercalator-Rh—2000 µM DescriptionA cationic nucleic acid intercalator that is used in CyTOF® analysis to discriminate dead cells from live cells or single nucleated cells from doublets.
201192A Cell-ID™ Intercalator-Ir—125 µM DescriptionA cationic nucleic acid intercalator that contains natural-abundance iridium (191Ir and 193Ir), which is used in CyTOF® analysis to discriminate dead cells from live cells or single nucleated cells from doublets.
201192B Cell-ID™ Intercalator-Ir—500 µM DescriptionA cationic nucleic acid intercalator that contains natural-abundance iridium (191Ir and 193Ir), which is used in CyTOF® analysis to discriminate dead cells from live cells or single nucleated cells from doublets.
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Maxpar Panel Kits
Simplify your study and save time by using preconfigured screening panels for comprehensive phenotyping and probing functionality of defined cell populations. Each predesigned panel is optimized with proven cellular markers and high-performing antibody combinations to maximize performance.
- Standard Panels
Product # Product name 201302 Maxpar® Human PB Basic I Phenotyping Panel Kit, 7 Marker—25 Tests DescriptionThis kit is designed to allow for the identification of CD4+ T, CD8+ T, B cells, monocytes, and granulocytes.
201303 Maxpar® Mouse Sp/LN Basic Phenotyping Panel Kit, 6 Marker—25 Tests DescriptionContains 6 antibodies and allows for the identification of CD4+ T, CD8+ T, B cells, macrophages, and monocytes.
201305 Maxpar® Human T-Cell Phenotyping Panel Kit, 16 Marker—25 Tests DescriptionAllows identification of major T cell subsets including naïve, central memory, effector, and effector memory cells, and classification of the activation and homing status of these subtypes.
201304 Maxpar® Human PB Phenotyping Panel Kit, 17 Marker—25 Tests DescriptionContains 17 antibodies for comprehensive identification of all major peripheral blood cellular subsets.
201306 Maxpar® Mouse Sp/LN Phenotyping Panel Kit, 16 Marker—25 Tests DescriptionDesigned with 16 antibodies to allow for the identification of all major mouse spleen/lymph cellular subsets.
201307 Maxpar® Human T-Cell Phenotyping EX Panel Kit, 10 Marker—25 Tests DescriptionThis kit, when combined with the Human T Cell Kit, allows for comprehensive identification of all major T cell subsets and classification of the activation and homing status of these subtypes.
201308 Maxpar® Human Intracellular Cytokine I Panel Kit, 11 Marker—25 Tests DescriptionDetects 11 major cytokines including cytolytic proteins granzyme B and perforin.
201309 Maxpar® Signaling I Panel Kit, 7 Marker—25 Tests DescriptionFor quantification of basal and induced phosphorylation of multiple key signaling pathways: JAK/STAT, NFκB, and MAPK.
201310 Maxpar® Mouse Intracellular Cytokine I Panel Kit, 8 Marker—25 Tests DescriptionContains all the reagents needed for CyTOF® measurement of 8 major mouse cytokines.
201311 Maxpar® Human HSPC PhenoEXPanel Kit, 7 Marker— 25 Tests DescriptionFor the identification and phenotyping of human hematopoietic progenitor populations.
201313 Maxpar® Cell Cycle Panel Kit, 5 Marker—25 Tests DescriptionThis kit is for assessment of cell cycle status: proliferation, G0, G1, S phase, G2, and M phase.
201314 Maxpar® Human B-Cell Phenotyping Panel Kit, 12 Marker— 25 Tests DescriptionFor the phenotyping of human B cells, including naïve, memory, transitional, and plasma B cell populations.
201315 Maxpar® Human PB Basic II Phenotyping Panel Kit, 7 Marker—25 Tests DescriptionDesigned to allow for the identification of CD4+ T, CD8+ T, B cells, monocytes, and granulocytes. The CD4/8 antibody clones distinguish Panel II from Panel I.
201316 Maxpar® Human AML Phenotyping Panel Kit, 15 Marker—25 Tests DescriptionFor the identification and phenotyping of human acute myeloid leukemia (AML).
201317 Maxpar® Human Mono/Macro Phenotyping Panel Kit, 15 Marker—25 Tests DescriptionIdentifies and phenotypes monocytes and macrophages, including CD14 and CD16.
201318 Maxpar® Human Helper T Cell Phenotyping Panel Kit, 15 Marker—25 Tests DescriptionFor the phenotyping of human CD4+ helper T cells including Th1, Th2, Th17, Th22, T follicular helper (TFH), and T regulatory (Treg) cells.
201319 Maxpar® Human Treg Phenotyping Panel Kit, 13 Markers—25 Tests DescriptionAllows identification and phenotyping of regulatory T cells based on expression factor Foxp3, CD25, and CTLA-4, along with low expression of the IL-7Rα chain (CD127).
201320 Maxpar® Human ES/iPS Phenotyping Panel Kit—25 Tests DescriptionMeasures the expression of the transcription factors Oct-3/4, Klf4, Sox2, and c-Myc (OKSM) to identify induced pluripotent stem (iPS) cells.
201321 Maxpar Human T Cell Immuno-Oncology EX Panel Kit, 8 marker—25 Tests DescriptionThe kit is designed to detect eight checkpoint and activation markers commonly expressed on activated T cells in oncology settings.
201322 Maxpar Complete Human T Cell Immuno-Oncology Panel Set—25 Tests DescriptionOne each of the Maxpar® Human T Cell Immuno-Oncology EX Panel Kit, Maxpar Human T Cell Phenotyping Panel Kit and Maxpar Human T Cell Phenotyping EX Panel Kit.
201323 Maxpar Basic Human T Cell Immuno-Oncology Panel Set—25 Tests DescriptionOne Maxpar® Human T Cell Immuno-Oncology EX Panel Kit and one Maxpar Human T Cell Phenotyping Panel Kit.
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Maxpar Direct Assays
The Maxpar® Direct™ Immune Profiling Assay™ with Maxpar Pathsetter™ software is the first complete sample-to-answer solution for high-dimensional immune profiling of human PBMC and whole blood.
Easily quantify 37 immune cell populations using a simple single-tube workflow with automated 5-minute results reporting.
Explore - Standard Panels
Product # Product name 201325 Maxpar® Direct™ Immune Profiling Assay™ DescriptionThe Maxpar Direct Immune Profiling Assay is designed and optimized for deep immune profiling of human peripheral whole blood and PBMC.
201402 Maxpar® Direct™ Myeloid and B Cell Expansion Panel 1 DescriptionMaxpar® Direct™ Myeloid and B Cell Expansion Panel 1 enables deeper phenotyping of myeloid and B cell subsets in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.
201403 Maxpar® Direct™ Myeloid and B Cell Expansion Panel 2 DescriptionMaxpar® Direct™ Myeloid and B Cell Expansion Panel 2 enables deeper phenotyping of B cell and myeloid cell subsets in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.
201404 Maxpar® Direct™ NK Cell Expansion Panel 1 DescriptionThe Maxpar® Direct™ NK Cell Expansion Panel 1 enables deeper phenotyping of NK cells in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.
201405 Maxpar® Direct™ T Cell Expansion Panel 1 DescriptionMaxpar® Direct™ T Cell Expansion Panel 1 enables phenotyping of T cell and NK cell exhaustion in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.
201406 Maxpar® Direct™ T Cell Expansion Panel 2 DescriptionMaxpar® Direct™ T Cell Expansion Panel 2 enables investigation of T cell migration, activation and exhaustion in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.
201407 Maxpar® Direct™ T Cell Expansion Panel 3 DescriptionMaxpar® Direct™ T Cell Expansion Panel 3 enables phenotyping of T cell costimulation and exhaustion in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.
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Maxpar Metal-Labeling Kits
With Maxpar metal-labeling kits you can tag IgG clones with your choice of up to 42 metals. Order them individually or as sets, with each kit containing enough material to conjugate 4–40 antibodies in 100 μg amounts.
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Multimetal Labeling Kits
The Multimetal Maxpar Kit contains a total of 40 reactions for conjugating metals to monoclonal antibodies, with up to 10 different metals of your choice (each at 4 reactions).
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Buffers and Solutions
Maxpar buffers provide optimal and consistent staining conditions for external, cytosolic and nuclear targets, while calibration beads enhance data quality and support intersample analysis.
- Reagents
Product # Product name 201063 Maxpar® Nuclear Antigen Staining Buffer Set—120 Tests DescriptionFormulated for staining antigens located within the nucleus, including transcription factors.
201065 Maxpar® Fix I Buffer—50 mL DescriptionOptimized for fixation of cells prior to permeabilization with the Maxpar Permeabilization Buffer when performing intracellular staining of antigens located in the cytoplasm and secretory pathway.
201066 Maxpar® Perm-S Buffer—250 mL DescriptionOptimized for permeabilization of cells following cell fixation with the Maxpar Fix I Buffer when performing intracellular staining of antigens located in the cytoplasm and secretory pathway.
201067 Maxpar® Fix and Perm Buffer—100 mL DescriptionOptimized for a single-step cell fixation, permeabilization, and intercalation. It also facilitates short-term storage of CyTOF® stained samples prior to analysis.
201068 Maxpar® Cell Staining Buffer—500 mL DescriptionThis buffer is intended for antibody and cell dilution steps and as a wash buffer during staining of cells for CyTOF® analysis.
201069 Maxpar® Water—500 mL DescriptionIntended for use as both a wash and a cell re-suspension solution during staining and preparation of cells for CyTOF® analysis.
201070 Washing Solution, E-Pure—1L DescriptionA solution containing trace amounts of hydrofluoric acid (HFA) used to wash out the sample line
201071 Washing Solution, E-Pure—250 mL DescriptionA solution that contains trace amounts of hydrofluoric acid (HFA) used to wash out the sample line prior to running samples
201072 Tuning Solution—250 mL DescriptionA high-purity standard solution containing known quantities of elements for the purpose of calibration of the instrument.
201078 EQ™ Four Element Calibration Beads—100 mL DescriptionA solution of polystyrene bead standards containing known concentrations of the metal isotopes 140/142Ce, 151/153Eu, 165Ho, and 175/176Lu used to normalize mass cytometry data.
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Custom Panels
Optimize your panel using the web-based Maxpar Panel Designer app, an online tool for building panels with Fluidigm metal-conjugated antibodies or custom conjugates. Panel Designer calculates signal overlap and utilizes a specialized algorithm to adjust metal-tagged targets to maximize performance. Export and share panels with collaborators or save for future import into our CyTOF systems.
Design -
Custom Antibody Conjugation
When your study calls for new antibodies or specific antibody configurations, consult our team of experts for custom conjugation services to tailor your antibody panels for collecting high-impact data. Our custom conjugation services speed you from design to discovery with made-to-order metal labeling for antibodies not currently listed in the Maxpar catalog, or alternative metal tagging for all listed items. Choose from 42 non-overlapping pure metal tags.
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Parts and Accessories
Find air filters, O-rings, torch assemblies, vacuum pump oil and more in our online catalog. It’s your digital source for replacement parts and product refills for all CyTOF systems.
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Therapeutic Insights Services
Learn how our expert consultants can work with you to tailor and execute a sample-to-answer project designed for your specific needs for single-cell mass cytometry or Imaging Mass Cytometry™ data.
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