Mass Cytometry

The kit enables unique barcoding of 20 samples so they can be combined and subsequently stained and acquired as one multiplexed sample, followed by software debarcoding and individual sample analysis.

Cell-ID Cisplatin specifically identifies dead cells when incubated prior to fixation or identifies total cells when incubated following cell fixation and permeabilization. Containing a mixture of naturally occurring platinum isotopes, it is best detected in the 195 channel.

Cell-ID™ Cisplatin-194Pt is a monoisotopic preparation of cisplatin-containing the 194Pt isotope. Cisplatin is a stain that binds covalently to cellular proteins and labels cells with compromised cell membranes to a greater extent than live cells. Cell-ID Cisplatin-194Pt therefore specifically identifies dead cells if cells are incubated prior to fixation, or it identifies total cells if cells are incubated following cell fixation and permeabilization. Because cisplatin binds covalently to protein, its labeling remains strong through subsequent cell handling steps used in downstream mass cytometry cell staining protocols.

Cell-ID™ Cisplatin-198Pt is a monoisotopic preparation of cisplatin-containing the 198Pt isotope. Cisplatin is a stain that binds covalently to cellular proteins, and labels cells with compromised cell membranes to a greater extent than live cells. Cell-ID Cisplatin-198Pt therefore specifically identifies dead cells if cells are incubated prior to fixation, or it identifies total cells if cells are incubated following cell fixation and permeabilization. Because cisplatin binds covalently to protein, its labeling remains strong through subsequent cell handling steps used in downstream mass cytometry cell staining protocols.

Cell-ID 127 IdU incorporates into DNA of proliferating cells and is a marker of S phase of the cell cycle.

A cationic nucleic acid intercalator that is used in CyTOF® analysis to discriminate dead cells from live cells or single nucleated cells from doublets.

A cationic nucleic acid intercalator that is used in CyTOF® analysis to discriminate dead cells from live cells or single nucleated cells from doublets.

A cationic nucleic acid intercalator that contains natural-abundance iridium (191Ir and 193Ir), which is used in CyTOF® analysis to discriminate dead cells from live cells or single nucleated cells from doublets.

A cationic nucleic acid intercalator that contains natural-abundance iridium (191Ir and 193Ir), which is used in CyTOF® analysis to discriminate dead cells from live cells or single nucleated cells from doublets.

This kit is designed to allow for the identification of CD4+ T, CD8+ T, B cells, monocytes, and granulocytes.

Contains 6 antibodies and allows for the identification of CD4+ T, CD8+ T, B cells, macrophages, and monocytes.

Allows identification of major T cell subsets including naïve, central memory, effector, and effector memory cells, and classification of the activation and homing status of these subtypes.

Contains 17 antibodies for comprehensive identification of all major peripheral blood cellular subsets.

Designed with 16 antibodies to allow for the identification of all major mouse spleen/lymph cellular subsets.

This kit, when combined with the Human T Cell Kit, allows for comprehensive identification of all major T cell subsets and classification of the activation and homing status of these subtypes.

Detects 11 major cytokines including cytolytic proteins granzyme B and perforin.

For quantification of basal and induced phosphorylation of multiple key signaling pathways: JAK/STAT, NFκB, and MAPK.

Contains all the reagents needed for CyTOF® measurement of 8 major mouse cytokines.

For the identification and phenotyping of human hematopoietic progenitor populations.

This kit is for assessment of cell cycle status: proliferation, G0, G1, S phase, G2, and M phase.

For the phenotyping of human B cells, including naïve, memory, transitional, and plasma B cell populations.

Designed to allow for the identification of CD4+ T, CD8+ T, B cells, monocytes, and granulocytes. The CD4/8 antibody clones distinguish Panel II from Panel I.

For the identification and phenotyping of human acute myeloid leukemia (AML).

Identifies and phenotypes monocytes and macrophages, including CD14 and CD16.

For the phenotyping of human CD4+ helper T cells including Th1, Th2, Th17, Th22, T follicular helper (TFH), and T regulatory (Treg) cells.

Allows identification and phenotyping of regulatory T cells based on expression factor Foxp3, CD25, and CTLA-4, along with low expression of the IL-7Rα chain (CD127).

Measures the expression of the transcription factors Oct-3/4, Klf4, Sox2, and c-Myc (OKSM) to identify induced pluripotent stem (iPS) cells.

The kit is designed to detect eight checkpoint and activation markers commonly expressed on activated T cells in oncology settings.

One each of the Maxpar® Human T Cell Immuno-Oncology EX Panel Kit, Maxpar Human T Cell Phenotyping Panel Kit and Maxpar Human T Cell Phenotyping EX Panel Kit.

One Maxpar® Human T Cell Immuno-Oncology EX Panel Kit and one Maxpar Human T Cell Phenotyping Panel Kit.

The Maxpar Direct Immune Profiling Assay is designed and optimized for deep immune profiling of human peripheral whole blood and PBMC.

Maxpar® Direct™ Myeloid and B Cell Expansion Panel 1 enables deeper phenotyping of myeloid and B cell subsets in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.

Maxpar® Direct™ Myeloid and B Cell Expansion Panel 2 enables deeper phenotyping of B cell and myeloid cell subsets in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.

The Maxpar® Direct™ NK Cell Expansion Panel 1 enables deeper phenotyping of NK cells in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.

Maxpar® Direct™ T Cell Expansion Panel 1 enables phenotyping of T cell and NK cell exhaustion in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.

Maxpar® Direct™ T Cell Expansion Panel 2 enables investigation of T cell migration, activation and exhaustion in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.

Maxpar® Direct™ T Cell Expansion Panel 3 enables phenotyping of T cell costimulation and exhaustion in conjunction with the Maxpar® Direct™ Immune Profiling Assay™.

Formulated for staining antigens located within the nucleus, including transcription factors.

Optimized for fixation of cells prior to permeabilization with the Maxpar Permeabilization Buffer when performing intracellular staining of antigens located in the cytoplasm and secretory pathway.

Optimized for permeabilization of cells following cell fixation with the Maxpar Fix I Buffer when performing intracellular staining of antigens located in the cytoplasm and secretory pathway.

Optimized for a single-step cell fixation, permeabilization, and intercalation. It also facilitates short-term storage of CyTOF® stained samples prior to analysis.

This buffer is intended for antibody and cell dilution steps and as a wash buffer during staining of cells for CyTOF® analysis.

Intended for use as both a wash and a cell re-suspension solution during staining and preparation of cells for CyTOF® analysis.

A solution containing trace amounts of hydrofluoric acid (HFA) used to wash out the sample line

A solution that contains trace amounts of hydrofluoric acid (HFA) used to wash out the sample line prior to running samples

A high-purity standard solution containing known quantities of elements for the purpose of calibration of the instrument.

A solution of polystyrene bead standards containing known concentrations of the metal isotopes 140/142Ce, 151/153Eu, 165Ho, and 175/176Lu used to normalize mass cytometry data.