Mass Cytometry

Driving groundbreaking discoveries one cell at a time, millions of times

Mass cytometry, powered by CyTOF® technology , is the world’s most advanced single-cell proteomics technology. It has opened new doors to discovery by empowering researchers to interrogate more than 50 markers simultaneously on millions of individual cells, to unveil new cell types, functions and biomarkers in immunology, cancer, stem cells and more.

Fluidigm Maxpar® reagents and protocols along with our Maxpar Panel Designer tool provide all you need for groundbreaking and cost-effective experiments that reveal systems-level biology at single-cell resolution.

New to CyTOF?

See how it compares to fluorescence cytometry.

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What's New

Flow Cytometer Trade-In Program

For a limited time, trade in any flow cytometer for up to a 30% discount on the purchase of a Helios™.

Switch to single-tube cytometry to accelerate your research with the most comprehensive view of cell phenotype and function.

LEARN MORE ABOUT TRADING IN

Explore Mass Cytometry

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Publications

Insights Powered by Mass Cytometry: Investigators Share Their Experience

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Mass Cytometry News

Download the List of 100+ Clinical Trials

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Use of CyTOF in Clinical Trials

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Standardized Immune Profiling: A Single-Tube Workflow with CyTOF

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FCS Express Analysis Tool for Mass Cytometry Data

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Cadmium-labeled CD45 Antibodies for Live Cell Barcoding

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Flexible Panel Design with Cadmium Metal Tags

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Methods

Sample Types

Current CyTOF systems, including the latest mass cytometer, Helios™, provide high-parametric data on individual cells prepared in liquid suspension. Standard protocols for preparing single-cell suspensions from tissue culture and solid and liquid tissues as well as tumors can be used for CyTOF sample prep. Sample size can vary from thousands to millions of cells.

Metal-Tagged Reagents

Cellular targets in liquid suspension can be labeled with more than 50 metal-tagged reagents, including antibodies, nucleic acid intercalators and analogs, as well as other biochemical ligands. Each reagent is detected and quantified with cytometry by time-of-flight mass spectrometry in the CyTOF system. The high purity and choice of metal isotopes ensure minimal background noise from signal overlap or endogenous cellular components. Our catalog is stocked with over 800 antibodies detecting more than 400 unique human or mouse targets. If a desired antibody is not yet present in our catalog, you can make it yourself using our metal-labeling kits or custom order biologically validated material using our custom conjugation services.

Cell Profiling

Targets can be anywhere in the cell, including the surface, cytoplasm, nucleus or internal organelles. Complete profiling allows CyTOF researchers to investigate a range of applications, including

Phosphoprotein detection can be used to map the signaling profile of samples, and optimized protocols and detailed technical assistance are available for any combination of targets.

Sample Barcoding

Sample barcoding is a powerful tool offering increased throughput, improved protocol consistency and enhanced data quality. Barcoding also enables efficient use of small precious samples through multiplexing and may provide cost savings through reduced consumption of reagents. Two approaches are available for CyTOF:

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