Changing the course of how diseases are treated and ultimately cured requires a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment. Imaging Mass Cytometry™ (IMC™) performed on the Hyperion™ Imaging System using Maxpar® metal-tagged antibodies empowers simultaneous interrogation of 4 to 37 protein markers at a time. It is now possible to deeply interrogate tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cellular morphology to uncover new biomarker correlations and cell interactions.
The Hyperion Imaging System provides highly multiplexed data with subcellular spatial resolution from 4 to 37 protein markers on FFPE or frozen tissues or cell smears. Standard protocols for preparing FFPE or frozen tissue slices can be used for sample prep for use with the system.
A common antibody retrieval protocol
The Maxpar antibody staining protocol for Imaging Mass Cytometry makes it easy to combine multiple metal-labeled antibodies in one step. Metal-tagged pathologist-verified Maxpar antibodies for Imaging Mass Cytometry are designed and optimized for use with the Hyperion Imaging System. These antibodies can be combined using a protocol that provides a common antigen retrieval step to simplify panel design for use with FFPE human tissue sections.
A precisely directed laser beam focused at 1 µm collects protein markers stained with metal-tagged Maxpar antibodies and directs these metal tags for detection using proven CyTOF® technology. A discrete signal from each ionized metal tag is detected based on differences in mass instead of wavelength, at 1 Da resolution. The purity and choice of metal isotopes ensure minimal background noise from signal overlap or endogenous cellular components.
Developed for use with the Hyperion Imaging System, the 60-plus and ever-growing list of antibodies currently available in our catalog has been optimized to detect defining markers in immuno-oncology pathways and cancer and immune cell populations. Each lot of conjugated antibody is quality control-tested on tissue sections using the Hyperion Imaging System. If a desired antibody is not yet present in our catalog, you can conjugate it yourself using our metal-labeling kits or inquire about our custom conjugation services.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Maxpar Metal-Conjugated Antibodies
Simplify Imaging Mass Cytometry panel design with pathologist-verified Maxpar antibodies. The Maxpar catalog features more than 60 metal-labeled antibodies and continues to grow rapidly. This initial selection of antibodies focuses on immune and oncology targets to be used with FFPE. Combining the antibodies of interest into a panel is easy using a protocol that provides a common antigen retrieval step. This reduces the time to panel development and significantly expands possibilities for immune system and immuno-oncology research on the Hyperion Imaging System. Design your own custom panel to analyze immunity, cancer progression or cancer immunotherapy research targets.
Primary Antibodies Explore
Maxpar Labeling Kits
With Maxpar metal-labeling kits you can tag IgG clones with your choice of up to 35 metals. Order them individually or as sets, with each kit containing enough material to conjugate 4 antibodies in 100 μg amounts.
Maxpar Labeling Kits Explore
Multimetal Labeling Kits
The multimetal Maxpar kit contains a total of 40 reactions for conjugating metals to monoclonal antibodies, with up to 10 different metals of your choice (each at 4 reactions).
- Labeling Kits
Product # Product name 201300 Maxpar® X8 Multimetal Labeling Kit—40 RxnDescription
Lets you conjugate IgG antibodies with your choice of up to 10 metals, with each kit containing enough material to conjugate 40 antibodies in 100 µg amounts .
Buffers and Solutions
Maxpar buffers provide optimal and consistent staining conditions for external, cytosolic and nuclear targets.
Product # Product name 201058 Maxpar® PBS—500 mLDescription
Maxpar® PBS is optimized for diluting Maxpar Barcode Perm Buffer (10X) and Maxpar Fix I Buffer (5X) into a final concentration of 1X prior to barcoding samples.
201069 Maxpar® Water—500 mLDescription
Intended for use as both a wash and a cell re-suspension solution during staining and preparation of cells for CyTOF® analysis.
Custom Antibody Conjugation
When your study calls for new antibodies or specific antibody configurations, consult our team of experts for custom conjugation services to tailor your antibody panels for collecting high-impact data. Our custom conjugation services speed you from design to discovery with made-to-order metal labeling for antibodies not currently listed in the Maxpar catalog, or alternative metal tagging for all listed items. Choose from 37 nonoverlapping pure metal tags. Contact your local sales representative for more information.