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Consumables

Advanta™ RNA-Seq NGS Library Prep Kit

This kit enables the preparation of NGS-ready, dual-indexed, full-length cDNA libraries of poly(A) RNA utilizing total RNA isolated from human, mouse, or rat for subsequent sequencing analysis. The kit contains four 48.Atlas™ IFCs and includes sufficient 3′ and 5′ primer indexes, reagents for generating strand-specific cDNA libraries, and Control Line Fluid for up to 48 samples (up to 6 libraries) per IFC. The 4 IFCs can accommodate a total of 192 samples (up to 24 libraries).


Kit Contents

  • 4 IFCs—48.Atlas (101-9303)
  • 8 syringes—Control Line Fluid (101-9307, 150 μL each)
  • Reagents in four boxes: Module 1, Module 2, Module 3, and Module 4

Advanta RNA-Seq NGS Library Prep Kit, Module 1: (–80 °C, 101-9299)

  • TSO Mix—1 tube with 50 μL (101-9091)

Advanta RNA-Seq NGS Library Prep Kit, Module 2: (–20 °C, 101-9223)

  • 1 tube DNA Polymerase—130 μL (101-9096)
  • 1 tube Enhancer Buffer—100 μL (101-9101)
  • 2 (of 4) bottles of Harvest Reagent Type 1—3.7 mL each (101-9300)
  • 1 tube Loading Reagent Type 1—200 μL (101-9301)
  • 1 tube mRNA Binding Buffer—1 mL (101-9079)
  • 1 tube N6—15 μL (101-9092)
  • 1 bottle PCR Buffer—3.5 mL (101-9097)
  • 1 tube PCR2 Primer—65 μL (101-9099)
  • 1 tube of Post-Capture Buffer—1 mL (101-9081)
  • 1 tube of Pre-Capture Buffer—120 μL (101-9080)
  • 1 tube qPCR Primer Premix—70 µL (101-9755)
  • 1 tube RNase Inhibitor—20 μL (101-9095)
  • 1 tube RT Buffer—50 μL (101-9093)
  • 1 tube RT Enzyme—24 μL (101-9094)
  • 1 tube Tris Buffer—1.25 mL (101-9098)

Advanta RNA-Seq NGS Library Prep Kit, Module 3: (–20 °C, 101-9302)

  • 1 bottle Nuclease-Free Water—5 mL (101-9100)
  • 2 (of 4) bottles Harvest Reagent Type 1—3.7 mL each (101-9300)

The 3’ PCR Primer HT Indexes:

  • 1 tube 3’ PCR Primer HT Index 1—15 µL (101-9102)
  • 1 tube 3’ PCR Primer HT Index 2—15 µL (101-9103)
  • 1 tube 3’ PCR Primer HT Index 3—15 µL (101-9104)
  • 1 tube 3’ PCR Primer HT Index 4—15 µL (101-9105)
  • 1 tube 3’ PCR Primer HT Index 5—15 µL (101-9106)
  • 1 tube 3’ PCR Primer HT Index 6—15 µL (101-9107)
  • 1 tube 3’ PCR Primer HT Index 7—15 µL (101-9108)
  • 1 tube 3’ PCR Primer HT Index 8—15 µL (101-9109)

The 5’ PCR Primer HT Indexes:

  • 1 tube 5’ PCR Primer HT Index 1—15 µL (101-9110)
  • 1 tube 5’ PCR Primer HT Index 2—15 µL (101-9111)
  • 1 tube 5’ PCR Primer HT Index 3—15 µL (101-9112)
  • 1 tube 5’ PCR Primer HT Index 4—15 µL (101-9113)
  • 1 tube 5’ PCR Primer HT Index 5—15 µL (101-9114)
  • 1 tube 5’ PCR Primer HT Index 6—15 µL (101-9115)
  • 1 tube 5’ PCR Primer HT Index 7—15 µL (101-9116)
  • 1 tube 5’ PCR Primer HT Index 8—15 µL (101-9117)
  • 1 tube 5’ PCR Primer HT Index 9—15 µL (101-9118)
  • 1 tube 5’ PCR Primer HT Index 10—15 µL (101-9119)
  • 1 tube 5’ PCR Primer HT Index 11—15 µL (101-9120)
  • 1 tube 5’ PCR Primer HT Index 12—15 µL (101-9121)

Advanta RNA-Seq NGS Library Prep Kit, Module 4: (4 °C, 101-9308)

  • mRNA Seq Beads—150 μL (101-9076)


Product insert available.

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