This kit enables the preparation of NGS-ready libraries from 27 exons and select intronic regions of the cystic fibrosis transmembrane conductance regulator (CFTR) gene for subsequent sequencing analysis. The kit includes sufficient targeted DNA sequencing library reagents, CFTR NGS Assay Pools, IFCs, and Control Line Fluid to generate 96 libraries. The kit also includes sufficient reagents and primers for target enrichment of DNA from samples with lower yield or DNA quality.
Each kit contains:
- 1 tube of TSP Sample Loading Reagent v2, 180 µL
- 1 tube of TSP Adapter Mix, 120 µL
- 1 tube of PCR Water, 1.8 mL
- 1 bottle of DNA Dilution Reagent, 3.0 mL
- 1 bottle of TSP Harvest Reagent, 10 mL
- 1 tube of TSP Assay Loading Reagent, 75 µL
- 1 tube of 4X TSP Master Mix, 210 µL
- 1 tube of TSP DNA Polymerase, 50 µL
- 1 set of Advanta™ CFTR NGS Assay Pools, 8 tubes per set, 10 µL/tube
- 2 IFCs, LP 48.48
- 4 syringes of LP Control Line Fluid (300 µL/syringe)
- 1 unit, Advanta™ NGS Preamp Reagent Kit—96 Rxn
- 1 unit, Advanta CFTR NGS Preamp Pool—96 Rxn (one tube, 60 uL/tube)
Product insert available.