|Wednesday, October 3, 2018 | 8:00 am PT, 11:00 am ET
|Kurt Schalper, MD, PhD
Assistant Professor of Pathology
Director, Translational Immuno-Oncology Laboratory
Yale Cancer Center
|Join us to learn about:
Visualize a new path forward
Changing the course of how diseases are treated and ultimately cured requires a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment. To meet this great challenge, we developed the Hyperion™ Imaging System.
The Hyperion Imaging System brings proven CyTOF® technology together with imaging capability to empower simultanous interrogation of 4 to 37 protein markers using Imaging Mass Cytometry™ (IMC™). Using this system, you can deeply interrogate tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cellular morphology to uncover new biomarker correlations and cell interactions. With the ability to utilize up to 135 channels to detect additional parameters, the Hyperion Imaging System is ideal to meet your image analysis needs today and well into the future.
Highly multiplexed immunohistochemistry (IHC) using proven CyTOF technology enables simultaneous analysis of 4 to 37 protein markers from a single scan
Identify new protein biomarkers and deeply profile tissue microenvironments at subcellular resolution while preserving the information in tissue architecture and cellular morphology
Preserve precious samples and reduce variability by eliminating dependency on serial sections
Hear what researchers are saying about Imaging Mass Cytometry and the Hyperion Imaging System:
Complete the form below to stay up to date on new Hyperion™ Imaging System antibodies, protocols and other developments.
Imaging Mass Cytometry (IMC) on the Hyperion Imaging System uniquely combines a precisely directed laser beam focused at 1 μm to collect biological samples stained with metal-tagged Maxpar® antibodies and directs these tags for analysis with proven CyTOF technology. Bringing together the high-multiplex capabilities of mass cytometry with imaging using metal-tagged antibodies allows simultaneous interrogation of 4 to 37 protein markers in tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cell morphology. Now researchers can detect discrete signals from each ionized metal tag based on differences in mass instead of wavelength at 1 Da resolution, and in contrast to fluorescence results, get uniform staining with minimal background.
Image 4 to 37 protein markers at subcellular resolution using the Hyperion Imaging System.
Hyperion Imaging System Support
Browse frequently asked questions, download technical documents, review training programs and service plans, find the right kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
IFCs, Assays and Reagents
Maxpar Metal-Conjugated Antibodies For Imaging Mass Cytometry
Simplify Imaging Mass Cytometry™ panel design with pathologist-verified Maxpar® antibodies optimized for use on the Hyperion™ Imaging System.
Maxpar Metal Labeling Kits
Multimetal Labeling Kit
The Multimetal Maxpar Kit contains a total of 40 reactions for conjugating metals to monoclonal antibodies with up to 10 different metals of your choice (each at 4 reactions).
Buffers And Solutions
Custom Antibody Conjugation Services
Custom conjugation services speed you from design to discovery with made-to-order metal labeling for antibodies not currently listed in the Maxpar Imaging Mass Cytometry catalog, or alternative metal tagging for all listed items.
The histoCAT™ software is an innovative computational Imaging Mass Cytometry™ analysis toolbox that enables comprehensive analysis of cellular phenotypes and their interrelationships within the spatial context of the tissue microenvironment. Developed in the laboratory of Bernd Bodenmiller at the University of Zurich, histoCAT includes novel algorithms to identify the relationships and “social networks” between cells in healthy and disease states and across experimental cohorts.
Under the agreement with the University of Zurich, Fluidigm obtained rights to globally distribute histoCAT along with the Hyperion™ Imaging System. Download
MCD™ Viewer is post-acquisition data processing software that allows users to visualize, review, and export Imaging Mass Cytometry™ data acquired with the Hyperion™ Imaging System and CyTOF® Software 6.7.
Hyperion Imaging System
|Mass range||75–209 amu|
|Abundance sensitivity||0.3% for 159Tb|
|Detection limit||≥400 copies per μm2|
|Dynamic range||4 orders of magnitude|
|Operating system||Windows® 7 Pro 64-bit|
|Data storage||>6 TB RAID (mirrored)|
|Switch time (between modes)||12 hr|
|Cross-cell contamination (redeposition)||≤2% (selected direction)|
|Crosstalk pixel to pixel||≤15% at 200 pixels/sec|
|Wet tissue thickness for full ablation||≤7 μm|
|Addressable sample size on slide||≥15 mm x 45 mm|
|Optical view of sample||≥ 250 μm x 250 μm|
|File type||TXT, multipage TIFF, OME-TIFF, MCD|
|Scan area||≥1 mm2/2 hr (@200 Hz)|
|Motherboard||HP® Z840 Workstation|
|CPU 1 and 2||Intel® Xeon® E5-2643 v4, 3.40 GHz, 2400 6C|
|RAM||64 GB DDR4-2400 (8 x 8 GB) 2 CPU RegRAM|
|Graphics card||NVIDIA® Quadro® K620 2 GB Graphics (DL-DVI+DP)|
|DVD||9.5 mm Slim SuperMulti DVD-RW 1st ODD|
|Keyboard||HP USB keyboard US|
|Mouse||HP USB optical mouse|
|Chassis||HP Z840 1,125 W (1,450 W/200 V) 90% efficient chassis|
|RAID controller||LSI 9270-8i SAS 6Gb/s ROC RAID Card|
|Storage drives||HP 2 x 256 GB SATA SSD RAID 1
Western Digital® 4 x 4 TB 7200 RPM SATA, RAID 10
|Monitor||LG® 34" Class 21:9 UltraWide® LED Monitor|
|Screen size||34.1 in (86.6 cm)|
Data File Size*
*1,000 x 1,000 μm region of interest (ROI)
Hyperion Tissue Imager Dimensions*
*The Hyperion Tissue Imager must be placed directly in front of the Helios instrument.
|Width||56 cm (22 in)|
|Height||134 cm (53 in)|
|Depth||56 cm (22 in)|
|Weight||159 kg (350 lb)|
|Width||103 cm (41 in)|
|Height||132 cm (52 in)|
|Depth||87 cm (35 in)|
|Weight||320 kg (705 lb)|
|Width||38 cm (15 in)|
|Height||64 cm (25 in)|
|Depth||67 cm (27 in)|
|Weight||81 kg (178 lb)|
|Width||83.05 cm (32.70 in)|
|Height||54.86 cm (21.60 in)|
|Depth||23.87 cm (9.40 in)|
|Weight||8.40 kg (18.50 lb) with stand|