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Hyperion Imaging System

A comprehensive system for highly multiplexed imaging

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Visualize a new path forward

Changing the course of how diseases are treated and ultimately cured requires a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment. To meet this great challenge, we developed the Hyperion™ Imaging System.

The Hyperion Imaging System brings proven CyTOF® technology together with imaging capability to empower simultaneous interrogation of 4 to 37 protein markers using Imaging Mass Cytometry™ (IMC™). Using this system, you can deeply interrogate tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cellular morphology to uncover new biomarker correlations and cell interactions. With the ability to utilize up to 135 channels to detect additional parameters, the Hyperion Imaging System is ideal to meet your image analysis needs today and well into the future.

Comprehensive

Highly multiplexed immunohistochemistry (IHC) using proven CyTOF technology enables simultaneous analysis of 4 to 37 protein markers from a single scan

Contextual

Identify new protein biomarkers and deeply profile tissue microenvironments at subcellular resolution while preserving the information in tissue architecture and cellular morphology

Powerful

Preserve precious samples and reduce variability by eliminating dependency on serial sections


Hear what researchers are saying about Imaging Mass Cytometry and the Hyperion Imaging System:

Learn more

Complete the form below to stay up to date on new Hyperion™ Imaging System antibodies, protocols and other developments.

Technology

Imaging Mass Cytometry (IMC) on the Hyperion Imaging System uniquely combines a precisely directed laser beam focused at 1 μm to collect biological samples stained with metal-tagged Maxpar® antibodies and directs these tags for analysis with proven CyTOF technology. Bringing together the high-multiplex capabilities of mass cytometry with imaging using metal-tagged antibodies allows simultaneous interrogation of 4 to 37 protein markers in tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cell morphology. Now researchers can detect discrete signals from each ionized metal tag based on differences in mass instead of wavelength at 1 Da resolution, and in contrast to fluorescence results, get uniform staining with minimal background.

Workflow

  1. Design
  2. Stain
  3. Image
  4. Analyze

Design

Design panels using pathologist-verified Maxpar antibodies conjugated to metal tags. See the current list of available antibodies here, use an antibody conjugation kit or contact your sales representative to inquire about our custom antibody conjugation service.

Stain

Maxpar antibodies for Imaging Mass Cytometry can be combined using a protocol that provides a common antigen retrieval step to simplify panel design for use with formalin-fixed, paraffin-embedded (FFPE) human tissue sections.

Image

Image 4 to 37 protein markers at subcellular resolution using the Hyperion Imaging System. 

Analyze

Analyze images in minutes using the MCD™ Viewer and easily export for secondary analysis on third-party software such as histoCAT™.

Support

Hyperion Imaging System Support

Browse frequently asked questions, download technical documents, review training programs and service plans, find the right kits and reagents for your research. Still need help? Reach out to one of our support specialists here.

components

IFCs, Assays and Reagents

Maxpar Metal-Conjugated Antibodies For Imaging Mass Cytometry

Simplify Imaging Mass Cytometry™ panel design with pathologist-verified Maxpar® antibodies optimized for use on the Hyperion™ Imaging System.

Maxpar Metal Labeling Kits

Conjugate metals to antibodies or other proteins in as little as two and a half hours with our Maxpar labeling kits, optimized for use with CyTOF systems.

Multimetal Labeling Kit

The Multimetal Maxpar Kit contains a total of 40 reactions for conjugating metals to monoclonal antibodies with up to 10 different metals of your choice (each at 4 reactions).    

Buffers And Solutions

Maxpar buffers provide optimal and consistent staining conditions for external, cytosolic and nuclear targets.

Custom Antibody Conjugation Services

Custom conjugation services speed you from design to discovery with made-to-order metal labeling for antibodies not currently listed in the Maxpar Imaging Mass Cytometry catalog, or alternative metal tagging for all listed items.

  1. Maxpar Metal-Conjugated Antibodies For Imaging Mass Cytometry
  2. Maxpar Metal Labeling Kits
  3. Multimetal Labeling Kit
  4. Buffers And Solutions
  5. Custom Antibody Conjugation Services

Software

CyTOF Software

CyTOF® Software v7.0 offers support for imaging and cell suspension-based sample analysis to Hyperion™ Imaging System and Helios™ system users. The software has the built-in capability to switch sample introduction modes, providing users the flexibility to configure hardware as required.

histoCAT

The histoCAT™ software is an innovative computational Imaging Mass Cytometry™ analysis toolbox that enables comprehensive analysis of cellular phenotypes and their interrelationships within the spatial context of the tissue microenvironment. Developed in the laboratory of Bernd Bodenmiller at the University of Zurich, histoCAT includes novel algorithms to identify the relationships and “social networks” between cells in healthy and disease states and across experimental cohorts.

Under the agreement with the University of Zurich, Fluidigm obtained rights to globally distribute histoCAT along with the Hyperion™ Imaging System. Download

MCD Viewer

MCD™ Viewer is post-acquisition data processing software that allows users to visualize, review, and export Imaging Mass Cytometry™ data acquired with the Hyperion™ Imaging System and CyTOF® Software v7.0.

  1. CyTOF Software
  2. histoCAT
  3. MCD Viewer

Specifications

Hyperion Imaging System

Channels 135
Mass range 75–209 amu
Abundance sensitivity 0.3% for 159Tb
Frequency 200 pixels/sec
Detection limit ≥400 copies per μm2
Dynamic range 4 orders of magnitude
Calibration Automated
Operating system Windows® 7 Pro 64-bit
Data storage >6 TB RAID (mirrored)
Switch time (between modes) 12 hr
Cross-cell contamination (redeposition) ≤2% (selected direction)
Crosstalk pixel to pixel ≤15% at 200 pixels/sec
Wet tissue thickness for full ablation ≤7 μm
Addressable sample size on slide ≥15 mm x 45 mm
Optical view of sample ≥ 250 μm x 250 μm
File type TXT, multipage TIFF, OME-TIFF, MCD
Scan area ≥1 mm2/2 hr (@200 Hz)

Workstation

Motherboard HP® Z840 Workstation
CPU 1 and 2 Intel® Xeon® E5-2643 v4, 3.40 GHz, 2400 6C
RAM 64 GB DDR4-2400 (8 x 8 GB) 2 CPU RegRAM
Graphics card NVIDIA® Quadro® K620 2 GB Graphics (DL-DVI+DP)
DVD 9.5 mm Slim SuperMulti DVD-RW 1st ODD
Keyboard HP USB keyboard US
Mouse HP USB optical mouse
Chassis HP Z840 1,125 W (1,450 W/200 V) 90% efficient chassis
RAID controller LSI 9270-8i SAS 6Gb/s ROC RAID Card
Storage drives HP 2 x 256 GB SATA SSD RAID 1

Western Digital® 4 x 4 TB 7200 RPM SATA, RAID 10

Monitor

Monitor LG® 34" Class 21:9 UltraWide® LED Monitor
Screen size 34.1 in (86.6 cm)

Data File Size*

*1,000 x 1,000 μm region of interest (ROI)

MCD 4-5 MB/channel
TXT 6-8 MB/channel

Hyperion Tissue Imager Dimensions*

*The Hyperion Tissue Imager must be placed directly in front of the Helios instrument.

Width 56 cm (22 in)
Height 134 cm (53 in)
Depth 56 cm (22 in)
Weight 159 kg (350 lb)

Helios Dimensions

Width 103 cm (41 in)
Height 132 cm (52 in)
Depth 87 cm (35 in)
Weight 320 kg (705 lb)

Chiller Size

Width 38 cm (15 in)
Height 64 cm (25 in)
Depth 67 cm (27 in)
Weight 81 kg (178 lb)

Monitor Size

Width 83.05 cm (32.70 in)
Height 54.86 cm (21.60 in)
Depth 23.87 cm (9.40 in)
Weight 8.40 kg (18.50 lb) with stand