Mass cytometry with Helios™ uses CyTOF® technology to enable deep profiling of translational and clinical research samples across a range of cell surface and intracellular markers.
The Helios mass cytometer uses the well-established technology of inductively coupled plasma mass spectrometry while handling sample preparation, sample introduction and data output in a manner similar to flow cytometry.
With mass cytometry, you can simultaneously interrogate cellular phenotype, function and signaling status in a single tube. Optional fixed- or live-cell barcoding improves data quality and workflow ease.
The use of heavy metal isotope tags to label cellular targets combined with the precision of mass spectrometry ensures background signals are negligible and signal overlap is minimized. For these reasons, designing high-dimensional panels is not constrained by the limitations of traditional fluorescence methods.
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Comprehensive
Accelerate insight into mechanisms of health and disease with the most comprehensive view of cell phenotype and function.
Powerful
Obtain the most actionable information from precious samples.
Proven
Trusted by translational and clinical study researchers around the world to power life-changing insights in human health
Hear what researchers are saying about the power of mass cytometry
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Technology
Mass cytometry uniquely combines time-of-flight mass spectrometry with Maxpar metal-labeling technology to enable breakthrough discovery and comprehensive functional profiling applications. Cellular targets are labeled with metal-tagged antibodies and detected and quantified by time-of-flight mass spectrometry. The high purity and choice of metal isotopes provide minimal background noise from signal overlap or endogenous cellular components. And 135 available detection channels ensure an ongoing ability to add more parameters, enabling you to fully study the functional complexity of biological systems at the single-cell level.
Stain cells using Fluidigm-validated protocols, buffers and barcoding reagents.
Acquire
Load prepared cells into Helios, which ionizes and releases the metal tags from individual cells, separates them by mass and counts the number bound to each cell.
Analyze
View and analyze high-dimensional data sets through a convenient cloud-based platform, Cytobank.
Support
CyTOF & Helios Support
Browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
Easily quantify 37 immune cell populations using a simple single-tube workflow with automated 5-minute results reporting. Learn more about the Maxpar Direct Immune Profiling Assay.
Maxpar Metal Labeling Kits
Conjugate metals to antibodies or other proteins in as little as two and a half hours with our Maxpar labeling kits, optimized for use with CyTOF systems.
Cell-ID Cell Labeling Reagent
Identify nucleated cells with dead cell discrimination and detect cells in S phase of the cell cycle with our Cell-ID labeling reagents.
Cell-ID 20-Plex Pd Barcoding Kit
Improve data quality, increase throughput and collect more exciting data with a set of unique palladium barcoding reagents and advanced debarcoding software.
Maxpar Sample Prep Buffers
Optimize your mass cytometry staining with our sample prep buffers, which can be used in surface, intracellular, nuclear and DNA staining protocols.
Maxpar Metal-Conjugated Antibodies
Achieve comprehensive functional and phenotypic profiling of cell samples with antibodies validated for functionality and compatibility.
Maxpar Panel Kits
Analyze a variety of proteins in cell populations with our ready-to-use kits containing up to 17 metal-conjugated antibodies. Areas of inquiry include stem-cell development, cell cycle, signaling and immunology.
Helios
Gain greater understanding of cell phenotypes, functions and relationships through comprehensive profiles of cells inside and out with the world’s most advanced mass cytometer.
Maxpar Direct Assays
Maxpar Metal Labeling Kits
Cell-ID Cell Labeling Reagent
Cell-ID 20-Plex Pd Barcoding Kit
Maxpar Sample Prep Buffers
Maxpar Metal-Conjugated Antibodies
Maxpar Panel Kits
Helios
Software
CyTOF Software
CyTOF® Software v7.0 offers support for imaging and cell suspension-based sample analysis to Hyperion™ Imaging System and Helios™ system users. The software has the built-in capability to switch sample introduction modes, providing users the flexibility to configure hardware as required.
Developed using probability state modeling, a proven high-parameter analytic approach, the software eliminates the variability of manual gating and provides an efficient and reliable solution for data analysis in longitudinal and multisite studies.
FCS Express
FCS Express™ is a fully integrated analysis, statistics, graphing and reporting tool for turning cytometry data into presentation-ready and publication-ready results. Full support for Fluidigm cytometry instruments ensures an enhanced analytical workflow and complete data acquisition-to-report experience for users.
New purchases of the Fluidigm Helios™ mass cytometer or Hyperion™ Imaging System will come with a free one-year license for FCS Express 7 Flow. Contact us to claim this offer.
Fluidigm Cytobank Analysis
Cytobank cloud-based data analysis software turns high-content data into high-impact knowledge by providing tools optimized for mass cytometry, including clustering (SPADE and viSNE), heat maps, dot plots, histograms and statistics, along with the ability to share your data with colleagues and collaborators.
CyTOF Software
Maxpar Pathsetter
FCS Express
Fluidigm Cytobank Analysis
Specifications
Helios System
Channels
135
Mass range
75–209 amu
Abundance sensitivity
0.3% for 159Tb
Instrument response
600,000 counts/pg 159Tb
Detection limit
350 antibodies/cell
Dynamic range
4.5 orders of magnitude
Calibration
Automated
Operating system
Windows® 7 Pro 64-bit
Data storage
7.2TB HDD RAID (mirrored)
Sample introduction
Pneumatic single tube loader with agitation, up to 5 ml volume
