Discover single-tube cytometry
Mass cytometry with Helios™ uses CyTOF® technology to enable deep profiling of translational and clinical research samples across a range of cell surface and intracellular markers.
The Helios mass cytometer uses the well-established technology of inductively coupled plasma mass spectrometry while handling sample preparation, sample introduction and data output in a manner similar to flow cytometry.
With mass cytometry, you can simultaneously interrogate cellular phenotype, function and signaling status in a single tube. Optional fixed- or live-cell barcoding improves data quality and workflow ease.
The use of heavy metal isotope tags to label cellular targets combined with the precision of mass spectrometry ensures background signals are negligible and signal overlap is minimized. For these reasons, designing high-dimensional panels is not constrained by the limitations of traditional fluorescence methods.
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Accelerate insight into mechanisms of health and disease with the most comprehensive view of cell phenotype and function.
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Mass cytometry uniquely combines time-of-flight mass spectrometry with Maxpar metal-labeling technology to enable breakthrough discovery and comprehensive functional profiling applications. Cellular targets are labeled with metal-tagged antibodies and detected and quantified by time-of-flight mass spectrometry. The high purity and choice of metal isotopes provide minimal background noise from signal overlap or endogenous cellular components. And 135 available detection channels ensure an ongoing ability to add more parameters, enabling you to fully study the functional complexity of biological systems at the single-cell level.
CyTOF & Helios Support
Browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
IFCs, Assays and Reagents
Maxpar Direct Assays
Easily quantify 37 immune cell populations using a simple single-tube workflow with automated 5-minute results reporting. Learn more about the Maxpar Direct Immune Profiling Assay.
Maxpar Metal Labeling Kits
Cell-ID Cell Labeling Reagent
Identify nucleated cells with dead cell discrimination and detect cells in S phase of the cell cycle with our Cell-ID labeling reagents.
Cell-ID 20-Plex Pd Barcoding Kit
Maxpar Sample Prep Buffers
Maxpar Metal-Conjugated Antibodies
Maxpar Panel Kits
CyTOF® Software v7.0 offers support for imaging and cell suspension-based sample analysis to Hyperion™ Imaging System and Helios™ system users. The software has the built-in capability to switch sample introduction modes, providing users the flexibility to configure hardware as required.
Maxpar® Pathsetter™ is a fully automated reporting and data analysis solution that automatically identifies 37 immune cell types in FCS files from samples processed with the Maxpar Direct Immune Profiling Assay.
Developed using probability state modeling, a proven high-parameter analytic approach, the software eliminates the variability of manual gating and provides an efficient and reliable solution for data analysis in longitudinal and multisite studies.
FCS Express™ is a fully integrated analysis, statistics, graphing and reporting tool for turning cytometry data into presentation-ready and publication-ready results. Full support for Fluidigm cytometry instruments ensures an enhanced analytical workflow and complete data acquisition-to-report experience for users.
New purchases of the Fluidigm Helios™ mass cytometer or Hyperion™ Imaging System will come with a free one-year license for FCS Express 7 Flow. Contact us to claim this offer.
Fluidigm Cytobank Analysis
Cytobank cloud-based data analysis software turns high-content data into high-impact knowledge by providing tools optimized for mass cytometry, including clustering (SPADE and viSNE), heat maps, dot plots, histograms and statistics, along with the ability to share your data with colleagues and collaborators.
|Mass range||75–209 amu|
|Abundance sensitivity||0.3% for 159Tb|
|Instrument response||600,000 counts/pg 159Tb|
|Detection limit||350 antibodies/cell|
|Dynamic range||4.5 orders of magnitude|
|Operating system||Windows® 7 Pro 64-bit|
|Data storage||7.2TB HDD RAID (mirrored)|
|Sample introduction||Pneumatic single tube loader with agitation, up to 5 ml volume|
|Peak throughput||2,000 (events/sec)|
|Flow rate||30–45 (µL/min)
|Replicate sample CV (normalized)||<3%|
|Height||52 in (132 cm)|
|Width||41 in (103 cm)|
|Depth||35 in (87 cm)|
|Weight||705 lb (320 kg)|
|Height||25 in (64 cm)|
|Width||15 in (38 cm)|
|Depth||27 in (69 cm)|
|Weight||178 lb (81 kg)|
|Operating system||Windows 7 Pro 64-bit|
|CPU||Intel® Core™ i7|
|Hard drives||2 x SSD, RAID 1|
|Data storage||7.2TB HDD, RAID 10|
|Monitor||24 in (61 cm) LED|
|Height||18 in (46 cm)|
|Width||8 in (20 cm)|
|Depth||23 in (58 cm)|
|Weight||77 lb (35 kg)|
Data File Size