High-Definition Biology
No more compromising for quality. With the Biomark™ HD system, you get reliable production-scale throughput and exquisite single-cell sensitivity, with a wide variety of sample types and chemistry choices to accommodate the most genomic applications. Now you can run entire projects from research to validation to routine screening on a single system with the flexibility you want and the consistency you need. Anything else we can help with?
Flexible
Design experiments to match your needs across the widest range of genomic applications.
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Sensitive
Detect unique molecular signatures in individual cells and rare cell populations.
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Fast
Accelerate discovery with a higher throughput and automated workflow.
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Efficient
Conserve precious samples—and funding—with lower reagent use and nanoliter reactions.
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Technology
Fluidigm’s revolutionary integrated fluidic circuits (IFCs) empower life
science research by automating PCR reactions in nanoliter volumes. This means
using less sample and reagent, and a single microfluidic device, to achieve the
high-quality, consistent results your work depends on. The Biomark HD system
runs IFCs in either real-time or end-point read modes, bringing flexible,
efficient and economical PCR solutions to a range of applications.
Workflow
- Prime
- Transfer
- Load
- Run
- Analyze
Prime
Prime the Dynamic Array IFC to prepare it for sample transfer.
Transfer
Pipet samples and primer-probe sets into the designated inlets on the IFC.
Load
Load the IFC on the controller, which prepares the reactions.
Run
Place the IFC into Biomark for thermal cycling and end-point or real-time
fluorescence detection.
Analyze
View and analyze color-coded expression heat maps, allele maps and cluster
plots for single or multiple runs with our analysis software.
Support
Biomark & EP1 Support
Explore tutorials and publications, browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
components
IFCs, Assays and Reagents
Digital Array IFCs
Ensure a precise determination of copy number variation with this innovative IFC designed specifically for digital PCR experiments.
Dynamic Array IFCs
Experience a new level of efficiency and throughput for gene expression and genotyping applications with the Fluidigm Dynamic Array™ IFCs used together with the Juno and Biomark HD systems.
SNP Type Assays
SNP Type™ assays provide a high-throughput, low-cost single-nucleotide polymorphism (SNP) genotyping solution enabling rapid assay design for efficient variant screening. The assays are based on allele-specific PCR SNP detection chemistry and are available for use with Fluidigm IFC technology on Biomark HD, EP1™ and Juno™ systems.
Delta Gene Assays
Realize the full potential of your Biomark™ HD system with minimal setup time and validated protocols that yield quality gene expression results. Just specify your genes of interest and target species to order our exclusive, high-quality, economical and MIQE-compliant Delta Gene™ assays (formerly DELTAgene). Designed specifically for use with Dynamic Array IFCs.
- Digital Array IFCs
- Dynamic Array IFCs
- SNP Type Assays
- D3 Assay Design
- Delta Gene Assays
Related Systems
IFC Controllers
Benefit from fewer steps and automated setup of our Dynamic Array™ and Digital Array™ IFCs with Fluidigm’s highly efficient IFC controllers.
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IFC Controllers
Software
Biomark Pathogen Detection Software v2.1.1
Biomark & EP1 Software
The latest software for the Biomark™ HD, Biomark and EP1™ systems. Package includes software for data collection, real-time PCR analysis, genotyping, digital PCR and melting curve analysis. To install, follow the instructions in the Updating the Biomark/EP1 Software Quick Reference (PN 101-6793).
IFC Controllers Software (HX, MX, RX, and WX)
The latest software for IFC Controllers HX, MX, RX and WX. To install, follow the instructions in the Update Software and Scripts section of the relevant IFC Controller User Guide (HX/MX, PN 68000112; RX, PN 100-3385; WX, PN 100-2297).
Singular Analysis Toolset Software
An innovative, open-source solution for analyzing and visualizing data, the Singular™ Analysis Toolset offers powerful methods for identifying gene expression and mutation patterns at the single-cell level. Designed for use with Biomark™and C1™ systems.
CopyCount-CNV Software
The cloud-based software analyzes raw fluorescence qPCR data from Biomark™ HD to determine absolute quantification and provide DNA copy number results. Applications include transgene copy number, event-sorting data analysis, and seed zygosity determination.
Each license supports one year of use from the date of activation and may be ordered through your Fluidigm account representative.
GO Immuno-Oncology Workbench
GO Immuno-Oncology Workbench is designed to further analyze primary data obtained with the Advanta™ IO Gene Expression Assay. GO Immuno-Oncology Workbench enables users to analyze large cohorts, discover population-level trends and accelerate checkpoint inhibitor research. It is designed by GenomOncology, a leader in the field of cancer molecular pathology analysis.
Each license supports one year of use from the date of activation and may be ordered through your Fluidigm account representative.
- Biomark Pathogen Detection Software v2.1.1
- Biomark & EP1 Software
- IFC Controllers Software (HX, MX, RX, and WX)
- Singular Analysis Toolset Software
- CopyCount-CNV Software
- GO Immuno-Oncology Workbench
Specifications
Instrument (Part Number BMKHD-BMKHD)
| Dimensions |
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| Depth |
75 cm (29.5 in) |
| Width |
75 cm (29.5 in) |
| Height |
100 cm (39.4 in) |
| Weight |
140 kg (308 lb) |
| Thermal Control |
Peltier-based, 4–99 ºC |
| Heating ramp rate |
Up to 5.5 ºC/sec |
| Cooling ramp rate |
Up to 5.5 ºC/sec |
| Detection |
CCD camera; 9M pixel, 12 μm x 12 μm pixel size |
| Fluorescence excitation |
475 nm, 530 nm, 575 nm |
| Fluorescence emission |
525 nm, 570 nm, 630 nm |
| Power requirements |
100–230 VAC, 50/60 Hz, 300 W
Fluidigm provides a region-specific power cord for the Biomark HD system. |
| Instrument Control Computer |
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| Computation |
Intel® Core™ i3-2120, 3.30 GHz |
| Memory |
2GB |
| Storage |
500 GB HDD |
| Ports |
4 USB
1GB/sec Ethernet |
| Operating System |
Microsoft® Windows® 10 IoT Enterprise 2016 LTSB (32 bit) |
| Monitor |
LCD flat panel |
| Accessories |
Keyboard and mouse |
Work Environment (indoor use only)
| Temperature |
15–30 ºC (59–86 ºF) |
| Humidity |
20%–80% relative humidity, non-condensing |
| Altitude |
Not to exceed 2,000 m (6,560 ft) above sea level |
Supported IFCs
| Gene expression |
48.48 Dynamic Array™ IFC
96.96 Dynamic Array IFC
192.24 Gene Expression IFC
Flex Six™ Gene Expression IFC |
| Genotyping |
48.48 Dynamic Array IFC
96.96 Dynamic Array IFC
192.24 Genotyping IFC
Flex Six Genotyping IFC |
| Digital PCR |
qdPCR 37K™ IFC
48.770 Digital Array™ IFC
12.765 Digital Array IFC |
Software
| Data Collection |
Biomark HD Data Collection software |
| Analysis |
Real-Time PCR Analysis software
SNP Genotyping Analysis software
Digital PCR Analysis software
Melt Curve Analysis software |
