Juno

NGS Library Preparation Workflow
Dispense the purified DNA, sample barcodes, PCR primers and master mix into designated inlets on the IFC before placing it onto the Juno loading tray.

NGS Library Preparation Workflow
Press Start. Juno automatically mixes nanoscale reactions within the IFC to amplify DNA targets and barcode sample libraries.

NGS Library Preparation Workflow
Targeted DNA sequencing libraries are collected from the IFC, and adapter PCR and cleanup are performed in a single tube reaction, followed by sequencing on an Illumina system.

SNP Genotyping Workflow
Dispense the purified DNA, sample barcodes, SNP Type™ or probe-based assays and master mix into designated inlets on the IFC before placing it onto the Juno loading tray.

SNP Genotyping Workflow
Press Start. Juno automatically mixes nanoscale PCR reactions within the IFC and performs amplification using the integrated thermocycler. For compatibility with low sample inputs down to 5.5 ng, the option for a low-cycle-number preamplification exists. This option is fully integrated and automated on the IFC.

SNP Genotyping Workflow
Transfer the IFC with completed SNP reactions to the Biomark HD or EP1™ for endpoint genotyping analysis.

Gene Expression Workflow
Dispense purified cDNA from RNA or miRNAs, qPCR assays and master mix into designated inlets on the IFC before placing it onto the Juno loading tray.

Gene Expression Workflow
Press Start. Juno automatically loads the IFC with input sample, assays and reagents to prepare for thousands of nanoscale real-time PCR reactions.

Gene Expression Workflow
Transfer the IFC with pre-mixed real-time PCR reactions to the Biomark HD for amplification and real-time quantitative detection.