Discover Your Inner Cell
Whether investigating therapeutic response in a human subject or a mouse model of disease, getting the most information from every precious sample is a priority in clinical and research studies alike.
Mass cytometry with Helios™ uses CyTOF® technology to enable deep profiling of translational and clinical research samples across a range of cell surface and intracellular markers.
Designed to significantly reduce signal overlap, mass cytometry empowers researchers to simultaneously interrogate cellular phenotypes, function and signaling status in higher dimension than traditional fluorescence methods.
Comprehensive
Accelerate insight into mechanisms of health and disease with the most comprehensive view of cell phenotype and function.
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Powerful
Obtain the most actionable information from precious samples.
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Proven
Trusted by translational and clinical study researchers around the world to power life changing insights in human health.
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Hear what researchers are saying about the power of mass cytometry
テクノロジー
Mass cytometry uniquely combines time-of-flight mass spectrometry with Maxpar metal-labeling technology to enable breakthrough discovery and comprehensive functional profiling applications. Cellular targets are labeled with metal-tagged antibodies and detected and quantified by time-of-flight mass spectrometry. The high purity and choice of metal isotopes provide minimal background noise from signal overlap or endogenous cellular components. And 135 available detection channels ensure an ongoing ability to add more parameters, enabling you to fully study the functional complexity of biological systems at the single-cell level.
ワークフロー
- Design
- Build
- Stain
- Acquire
- Analyze
Acquire
Load prepared cells into Helios, which ionizes and releases the metal tags from individual cells, separates them by mass and counts the number bound to each cell.
Analyze
View and analyze high-dimensional data sets through a convenient cloud-based platform,
Cytobank.
Support
CyTOF & Helios Support
Browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
構成
IFCs, Assays and Reagents
Maxpar Direct Assays
Easily quantify 37 immune cell populations using a simple single-tube workflow with automated 5-minute results reporting. Learn more about the Maxpar Direct Immune Profiling Assay.
Maxpar Metal Labeling Kits
Conjugate metals to antibodies or other proteins in as little as two and a half hours with our
Maxpar labeling kits, optimized for use with CyTOF systems.
Cell-ID™細胞標識試薬
Cell-IDに含まれる2種類の細胞染色試薬、インターカレーター-Irおよびインターカレーター-103Rhを使用して細胞の生死判定を行い、お使いのサンプルを確実にキャラクタリゼーションできます。
Cell-ID 20-Plex Pd Barcoding Kit
Improve data quality, increase throughput and collect more exciting data with a set of unique palladium
barcoding reagents and advanced debarcoding software.
Maxpar Sample Prep Buffers
Optimize your mass cytometry staining with our
sample prep buffers, which can be used in surface, intracellular, nuclear and DNA staining protocols.
Maxpar Metal-Conjugated Antibodies
Achieve comprehensive functional and phenotypic profiling of cell samples with
antibodies validated for functionality and compatibility.
Maxpar Panel Kits
Analyze a variety of proteins in cell populations with our
ready-to-use kits containing up to 17 metal-conjugated antibodies. Areas of inquiry include stem-cell development, cell cycle, signaling and immunology.
Helios
Gain greater understanding of cell phenotypes, functions and relationships through comprehensive profiles of cells inside and out with the world’s most advanced mass cytometer.
- Maxpar Direct Assays
- Maxpar Metal Labeling Kits
- Cell-ID™細胞標識試薬
- Cell-ID 20-Plex Pd Barcoding Kit
- Maxpar Sample Prep Buffers
- Maxpar Metal-Conjugated Antibodies
- Maxpar Panel Kits
- Helios
ソフトウェア
CyTOF Software
CyTOF® Software v7.0 offers support for imaging and cell suspension-based sample analysis to Hyperion™ Imaging System and Helios™ system users. The software has the built-in capability to switch sample introduction modes, providing users the flexibility to configure hardware as required.
Maxpar Pathsetter
Maxpar® Pathsetter™ is a fully automated reporting and data analysis solution that automatically identifies 37 immune cell types in FCS files from samples processed with the Maxpar Direct Immune Profiling Assay.
Developed using probability state modeling, a proven high-parameter analytic approach, the software eliminates the variability of manual gating and provides an efficient and reliable solution for data analysis in longitudinal and multisite studies.
- CyTOF Software
- Maxpar Pathsetter
- フリューダイムCytobank
仕様
Helios System
| Channels |
135 |
| Mass range |
75–209 amu |
| Abundance sensitivity |
0.3% for 159Tb |
| Instrument response |
600,000 counts/pg 159Tb |
| Detection limit |
350 antibodies/cell |
| Dynamic range |
4.5 orders of magnitude |
| Calibration |
Automated |
| Operating system |
Windows® 7 Pro 64-bit |
| Data storage |
7.2TB HDD RAID (mirrored) |
| Sample introduction |
Pneumatic single tube loader with agitation, up to 5 ml volume |
| Peak throughput |
2,000 (events/sec) |
| Flow rate |
30–45 (µL/min)
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| Replicate sample CV (normalized) |
<3% |
| Height |
52 in (132 cm) |
| Width |
41 in (103 cm) |
| Depth |
35 in (87 cm) |
| Weight |
705 lb (320 kg) |
Chiller
| Height |
25 in (64 cm) |
| Width |
15 in (38 cm) |
| Depth |
27 in (69 cm) |
| Weight |
178 lb (81 kg) |
Workstation
| Operating system |
Windows 7 Pro 64-bit |
| CPU |
Intel® Core™ i7 |
| Hard drives |
2 x SSD, RAID 1 |
| Data storage |
7.2TB HDD, RAID 10 |
| Monitor |
24 in (61 cm) LED |
| Height |
18 in (46 cm) |
| Width |
8 in (20 cm) |
| Depth |
23 in (58 cm) |
| Weight |
77 lb (35 kg) |
Data File Size
| IMD |
0.3 MB/sec/channel |
| FCS |
12.4 bytes/event/channel |
| TXT |
2.4 bytes/event/channel |
