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What sequencing depth is recommended for TraCeR analysis?

The data presented by Stubbington et al. (Nature Methods, 2016), who developed TraCeR, was sequenced with paired-end 100 bp reads, with a sequencing depth analysis suggesting that 1 million reads per cell were found to be sufficient to detect most TCR α and β chain sequences in the demonstrated experimental model.


For Research Use Only. Not for use in diagnostic procedures.