How quickly do I need to use the IFC after the foil pouch is opened?

IFCs must be used within 24 hours of opening the sealed foil pouch. The exception is the Flex Six IFC, which has a 90-day lifetime. However, the lifetime of a Flex Six IFC will decrease if it is not handled according to Fluidigm guidelines. For example, postponing the post-PCR script or altering thermal cycling conditions shortens the expected lifetime of a Flex Six IFC.