If I have multiple IFC runs, can I prepare my sample mix and assay mix the day before? A week before?
Sample mixes should be prepared the same day the IFC is loaded. Once prepared, never freeze and thaw sample mixes.
While the best practice is to prepare assay mixes just before use, they can be prepared in advance and stored at 4 °C for short periods or at –20 °C for long-term storage. Avoid multiple freeze-thaw cycles of assay mixes. Testing of stored assay mixes is recommended to ensure consistent performance.