Why can’t I load the samples in the assay inlets and the assays in the sample inlets?
The sample and assay chambers inside the IFC are different sizes. For this reason, the sample mix is prepared at a 1X concentration, whereas the assay mix is prepared at a 10X or 20X concentration, depending on the IFC used. Pipetting the sample and assay preparations into the incorrect inlets will result in incorrect concentrations for both in the final reaction.
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