How do the SNP Type and TaqMan chemistries differ?
Both chemistries are robust methods for SNP genotyping, and both are compatible with specific target amplification protocols. Key differences between the chemistries are highlighted in the table below:
| TaqMan® | SNP Type | |
|---|---|---|
| Mechanism | Allele-specific hybridization | Allele-specific extension |
| Primers | Locus-specific (2) | Sequence-tagged, allele-specific (2), and locus-specific (1) |
| Probes | FAM™, VIC® | FAM, HEX |
| Labeling | Individual | Universal |
| Fluorescence generation | Probe displacement and cleavage | Fluorescent amplicon formation |
| Fluorescence detection | Real-time or endpoint | Endpoint |
For more information regarding specific protocols and necessary reagent components, see the SNP Genotyping User Guide (PN 68000098) or the Juno System User Guide (PN 100-7070).
For Research Use Only. Not for use in diagnostic procedures.