Why is the Exo I treatment after preamplification needed only for EvaGreen?

EvaGreen® works by detecting all double-stranded DNA. Exo I treatment after the preamplification step removes unincorporated primers. If these primers are not removed, they can cause increased background in EvaGreen-based detection. Unincorporated primers do not contribute to background when using TaqMan® probes.

For Research Use Only. Not for use in diagnostic procedures.