How do you identify single cells? And how do you distinguish those from aggregates?
Samples are labeled with iridium nucleic acid intercalator. For each event, many features are recorded, including signal duration (called Event Length) and iridium intensity.
Single events have lower iridium intensity (since they have less DNA) and lower Event Length values compared to aggregates. These characteristics enable gating of single cells.
For Research Use Only. Not for use in diagnostic procedures.