Why do I need to prepare fresh 80% ethanol?

Diluted ethanol evaporates over time, and its concentration changes. To ensure that the protocol is performed correctly and that results are consistent, we recommend preparing fresh 80% ethanol prior to each extraction. To prevent evaporation, make sure to close the tubes after 80% ethanol is added, particularly when processing large batches (>10 samples in parallel).

For Research Use Only. Not for use in diagnostic procedures.