Single-cell genomics is rapidly transforming biological research through genome sequencing, transcriptomics and epigenetic profiling. See Fluidigm at Single Cell Genomics 2017 in Rehovot, Israel to learn about using the C1™ system for single-cell RNA, miRNA, DNA and epigenetic applications across a diverse range of cell types and nuclei.
Researchers characterizing the genetics and function of individual cells in their native environments are uncovering new insights. This understanding is revealing the need for establishing best practices and sampling standards; modeling and analysis; single-cell genomics and epigenomics integration; and immunology, cancer research and germ cell implications. To help define the single-cell genomics field, leading experts will convene at Single Cell Genomics 2017 for interdisciplinary discussion. The meeting will include presentations, posters and abstracts from dozens of luminaries, along with time for discussion and networking.
Presentations showing how Fluidigm technologies are powering advances in single-cell genomics:
- Analyzing cell diversity and characterizing cellular function with single-cell genomics using the C1 system
Mark Lynch, Senior Product Manager, Fluidigm Corporation
Since launching in 2013, the C1 system has contributed to the success of the now well-established single-cell mRNA sequencing application. In recent years, we’ve evolved with the single-cell community to build an extensive range of applications designed to equip researchers with new single-cell genomic technologies.
We’ll present a summary of single-cell genomic applications for C1 to show some ways you can use the platform for single-cell genomic analysis.
- Interrogating human intratumoral CD4 T cells by single-cell RNA-seq
Massimiliano Pagani, PhD, Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, Istituto Nazionale Genetica Molecolare, “Romeo ed Enrica Invernizzi,” Milan, Italy
Tumor-infiltrating regulatory T lymphocytes (Treg) can suppress effector T cells specific to tumor antigens. Deeper molecular definitions of tumor-infiltrating lymphocytes could thus offer therapeutic opportunities.
We performed single-cell transcriptomic analysis of more than 2,000 tissue-infiltrating Treg cells using the C1 platform. We’ll show that analysis of these data suggests underlying cellular heterogeneity that’s indiscernible at the population level. These findings illustrate the importance of studying Treg cells contextually at tumor sites to better elucidate the underlying mechanisms of localized immune responses and to define new potential targets for immune-based cancer therapies.
Posters showing how Fluidigm technologies are powering advances in single-cell genomics:
- Improved Single-Cell mRNA Sequencing for Transcriptome and Paired-Chain TCR Analysis of Primary Human CD3+ T Cells
Camila Egidio, Michael Gonzales, Joel Brockman, Shuwen Chen, Aik Ooi, Robert Durruthy-Durruthy, Clare Rogers, Manisha Ray
- Targeted Single-Cell Transcription Factor Profiling Outperforms mRNA-Seq for In-Depth Characterization of Cancer Cells
Camila Egidio, Robert Durruthy-Durruthy, Manisha Ray, Jason McKinney
- A High-Throughput System for Generating Exceptional-Quality mRNA-Seq Analysis on 800 Single Cells
Ilona Holcomb, Aik Ooi, Gajalakshmi Dakshinamoorthy, Joel Brockman, Benjamin Lacar, Nianzhen Li, Ramesh Ramakrishnan
Single Cell Genomics 2017 is fully booked. Complete the form below to receive copies of our Fluidigm abstracts and slide presentations.
For Research Use Only. Not for use in diagnostic procedures.