Publications

Publications

Single-cell mass cytometry of acute myeloid leukemia and leukemia stem/progenitor cells

Zeng, Z., Konopleva, M., Andreeff, M.

Mass cytometry time-of-flight (CyTOF®) empowers us to understand acute myeloid leukemia (AML) biology at the single-cell level. This technology, combined with advanced data-analysis methods, enables identification and characterization of the rare AML stem/progenitor cells that play key roles in drug resistance and AML relapse. Here we provide a protocol for AML sample preparation for CyTOF and keynotes emphasizing critical steps in and troubleshooting strategies for this procedure.

Citation

Zeng, Z., Konopleva, M., Andreeff, M. "Single-cell mass cytometry of acute myeloid leukemia and leukemia stem/progenitor cells" Methods in Molecular Biology (2017): 75–86