Informing vaccination strategies with identification of protective immunological features using mass cytometry
In this presentation, Marcelo Sztein, MD, shows examples of his group’s use of mass cytometry to help advance the fields of vaccine development and pathogen-host interactions. He provides an in-depth look at his studies of Salmonella typhi (S. typhi), the causative agent of typhoid fever, an infectious disease of great public health importance.
In this webinar you will learn how Sztein’s team successfully used mass cytometry to
- identify the key role that regulatory T cells appear to play in the development of typhoid disease following an oral challenge with wild-type S. typhi in humans
- investigate in detail the differences observed in T cell-mediated immunity elicited by oral immunization with the licensed Ty21a-attenuated typhoid fever vaccine in children and adults
- uncover early cell type-specific epigenetic modifications elicited in human gut cells following ex vivo exposure to S. typhi, which are likely to influence downstream immune responses in the gut mucosa microenvironment.
About the presenter:
Marcelo B. Sztein, MD
Center for Vaccine Development and Global Health
University of Maryland
This webinar first aired on September 23, 2020, at the 9th Annual Fluidigm Mass Cytometry Summit: Virtual.
- Rudolph, M.E. et al. “Age-associated heterogeneity of Ty21a-induced T cell responses to HLA-E restricted Salmonella typhi antigen presentation.” Frontiers in Immunology 10 (2019): 257.
- Sztein, M.B. et al. "Salmonella enterica serovar Typhi exposure elicits ex vivo cell-type-specific epigenetic changes in human gut cells." Scientific Reports 10 (2020): 13581.
- Booth, J.S. et al. "Effect of the live oral attenuated typhoid vaccine, Ty21a, on systemic and terminal ileum mucosal CD4+ T memory responses in humans." International Immunology 31 (2020): 101-116.
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For Research Use Only. Not for use in diagnostic procedures.