Immuno-Oncology

Reveal the molecular signatures of tumor immune response

Application

Highlights

Optimized
Screen high-value markers of the tumor immune response.


Flexible
Easily add new markers over time, customizing for your own research needs.


Efficient

Run 24 to 96 samples at a time using the robust Biomark™ HD automated qPCR system.

The Advanta™ IO Gene Expression Assay is a qPCR assay for expression of 170 genes that enables profiling of tumor immunobiology and new biomarker identification.

Designed to meet the rigorous demands of human checkpoint research programs, the Advanta IO Gene Expression Assay includes 91 key markers of tumor immune response that were shown in a multicenter international clinical trial to inform tumor progression and checkpoint therapeutic response1,2. Working with researchers in the biopharmaceutical industry, we further expanded the panel to include 74 additional immuno-oncology markers.

The two-panel set includes genes for identification and functional analysis of immune and cancer cells, including markers found in defined T cell subsets, cytokines and chemokines and markers of immune regulation and immune cell fate. The Advanta IO Gene Expression Assay is a key component of a complete workflow from sample to data supporting your research in tumor immune response.

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Panel A

ARG1 CLEC4C IL12A PDCD1LG2 (PD-L2)
BTLA CSF2 IL13 PRF1
CCL2 CTLA4 IL17A PTGER2
CCL22 CX3CL1 IL17F PTGER4
CCL28 CXCL10 IL1B PTGS2
CCR5 CXCL8 IL2 PTPRC
CCR7 CXCL9 IL2RA RORC
CD1C CXCR3 IL4 SDHA
CD244 EOMES IL6 SP2
CD27 EPCAM IL7 TBX21
CD274 (PD-L1) FOXP3 IL7R TGFB1
CD276 GZMA ITGAM TMEM55B
CD28 GZMB ITGAX TNF
CD3E HAVCR2 ITGB2 TNFRSF14
CD4 HLA-A KLRK1 TNFRSF4
CD40 HLA-B LAG3 TNFRSF9
CD40LG HLA-C LGALS9 TNFSF4
CD48 HMOX1 MAP4K1 TNFSF9
CD69 ICAM1 MICA VCAM1
CD70 ICOS MICB VEGFA
CD80 IDO1 MS4A1 VPS33B
CD86 IFNG NCAM1 VTCN1
CD8A IL10 PDCD1 (PD-1)

Panel B

APOBEC3A CXCR4 IFNA2 NKG7
APOBEC3B CYBB IGHA1 NRAS
ARG2 DGAT2 IGHG1 NT5E
CA4 EBI3 IGHM PYGL
CCL18 ERBB2 JCHAIN (IGJ) SLAMF7
CCL21 FASLG IGKC SLAMF8
CCL3 FCER1G IGLJ3 STAT1
CCL4 FCRLA IGSF6 STAT2
CCL5 FYB IL10RA STAT3
CD160 GATA3 IL12B STAT5A
CD19 GNLY IL15 STAT5B
CD1D GZMH IL2RG STAT6
CD2 GZMK IRF9 TLR7
CD22 HLA-DMB ISG15 TLR8
CD37 HLA-DPB1 JAK2 TNFAIP8
CD52 HLA-DQB1 KREMEN1 TNFRSF18
CD53 HLA-DRB1 LAPTM5 TNFSF18
CD63 IFI27 LCK
CTSS IFIT2 LRG1

Table 1. The Advanta IO Gene Expression Assay gene list by panel. Panel A includes 91 target genes and 5 housekeeping genes. Panel B includes 74 target genes and the same 5 housekeeping genes, along with 17 open assay inlets to facilitate the addition of custom content over time. Refer to Ordering information for the panel content of each component.


Complete workflow from sample to data analysis


Figure 1. Assay workflow. The gene expression assay workflow uses the Juno™ system for IFC preparation and the Biomark HD for qPCR thermal cycling and data acquisition. Generate 175–192 assay results per sample (up to 24 samples) in 4.5 hours with 1.3 hours of hands-on time using the GE 24.192 IFC. Alternatively, generate up to 96 assay results per sample (up to 96 samples) in 6.5 hours with 1.5 hours of hands-on time using the GE 96.96 IFC. After primary data analysis, results can be further analyzed with third-party software, such as GO Immuno-Oncology Workbench.

The Biomark HD advantage

The Advanta IO Gene Expression Assay is optimized to run on the industry-proven Biomark HD system. Leveraging the power of microfluidics, the system uses integrated fluidic circuits (IFCs) to precisely combine multiple reactions at nanoliter volumes. Enabling you to easily automate the gene expression workflow, Biomark HD delivers performance you can trust with minimal hands-on time.

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Figure 2. Manage sample throughput to meet your research needs with integrated fluidic circuits (IFCs). The Gene Expression 24.192 IFC (left) accommodates up to 24 samples per run. For laboratories with higher throughput needs, the Gene Expression 96.96 IFC (right) accommodates up to 96 samples per run. Respectively, the IFCs generate a total of 4,608 and 9,216 parallel reactions.

Replicates per Sample

Samples per GE 24.192 IFC

Samples per GE 96.96 IFC

1 24 96
2 12 48
3 8 32
4 6 24

Table 3. Sample capacity per IFC.  The number of samples that can be processed in a single run depends on the IFC format and the number of replicates per sample. 

Ordering information

Product Name Part Number
Advanta IO Gene Expression Assay—Panels A & B, GE 24.192, 2 IFCs 101-7678
Advanta IO Gene Expression Assay—Panel A, GE 96.96, 2 IFCs 101-6082
Advanta IO Gene Expression Assay—Panel B, GE 96.96, 2 IFCs 101-6083
Advanta IO Gene Expression Assay—Panels A & B, GE 96.96, 4 IFCs 101-6084
Recommended Products Sold Separately Part Number
Advanta IO Gene Expression Control 1 101-7676
Advanta FFPE RNA Extraction Kit 101-6773
GO Immuno-Oncology Workbench* 101-6819

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References
1. Fehrenbacher, L. et al. The Lancet 387 (2016): 1,837–1,846
2. Herbst, R.S. et al. Nature 515 (2014): 563–567

* GO Immuno-Oncology Workbench was developed by GenomOncology.

For Research Use Only. Not for use in diagnostic procedures.