Click the link below to view training tutorials, browse FAQs, read technical documents, review service plans and locate the right IFCs, kits and reagents for your research. If you still need help, reach out to one of our support specialists here.
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FAQs
We’ve answered some of the most relevant recurring questions about Juno™ systems to ensure that you have the information you need to succeed in your research. For additional information, check out the tutorials and technical documents or inquire about one of our training programs.
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Applications
- What materials are included in each kit?
- What information is available about the performance of the Advanta CFTR NGS Library Prep Assay?
- Can you provide a summary of the assay workflow?
- What data analysis and bioinformatic pipeline tools are available?
- What is the cost of the product and what is the effective cost per sample?
- How do I order the product?
- Can I evaluate the product prior to making a purchase commitment?
- How does this assay compare to competitor assays with regard to performance, cost and workflow, and what sets Fluidigm apart from the competition?
- Which makes and models of NGS systems are supported by the assay?
- What other assays are available for the Juno system and Access Array system?
- What equipment is required to use the product?
- How many samples can be processed per workday, and what is the total turnaround time from start to finish?
- How do I estimate read depth coverage?
- How many samples can be processed in a single batch?
- What are the sample requirements?
- Does your product include sample barcodes?
- Which CFTR variants does the Advanta CFTR NGS Library Prep Assay cover and how were they chosen?
- How many amplicons are generated by the Advanta CFTR NGS Library Prep Assay, and what is the size?
- Have you tested any materials that could be used as controls, and can you recommend a source for control materials?
- Can Fluidigm sequester specific lots of reagents and/or consumables for my lab, and if so, what is the process for sequestering lots?
- What materials are needed to use the kit but are not included in the kit?
- How many unique sample barcodes are available?
- Do you have any publications from users of the product?
- How did you handle the detection of the large exonic deletions within your design?
- Can I include sample identification markers such as the Fluidigm SNP Trace sample ID panel?
- Which sequencing systems are compatible with the Advanta NGS Library Prep Reagent Kit?
- What are the differences between Targeted DNA Seq Library Reagent Kits and Advanta NGS Library Prep Reagent Kits?
- What do I need to perform targeted DNA sequencing library preparation?
- What species and targets do you design assays to?
- What is the minimum DNA input for assays using the Advanta NGS Library Prep Reagent Kit?
- What methods are supported by the Juno instrument?
- Can I automate use of the product with my liquid handler?
- What level of manual input is required?
- What level of skill is required to perform the assay and how complex is the workflow?
- When is the use of preamplification recommended?
- Can I add other variants of interest that are not covered by the Advanta CFTR NGS Library Prep Assay?
- Your CF design only uses a portion of the assay inlets within the IFC. What am I supposed to do with the unused assay inlets? View more
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Software
- What data analysis and bioinformatic pipeline tools are available?
- How can I download the latest Juno instrument software?
- What is the difference between SNP Type Normalization and no template control (NTC) normalization?
- What is the difference between NO CALL and an Invalid call in the Fluidigm Genotyping Analysis software?
- What can I do to help improve call rates?
- How does the Fluidigm SNP Genotyping Analysis software determine the genotype call for a sample?
- When should I use the All SNP Scatter Plot View option in the SNP Genotyping Analysis software? View more
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Design
- What materials are included in each kit?
- What information is available about the performance of the Advanta CFTR NGS Library Prep Assay?
- How do I estimate read depth coverage?
- How many samples can be processed in a single batch?
- What are the sample requirements?
- Does your product include sample barcodes?
- Which CFTR variants does the Advanta CFTR NGS Library Prep Assay cover and how were they chosen?
- How many amplicons are generated by the Advanta CFTR NGS Library Prep Assay, and what is the size?
- Have you tested any materials that could be used as controls, and can you recommend a source for control materials?
- What materials are needed to use the kit but are not included in the kit?
- How many unique sample barcodes are available?
- How did you handle the detection of the large exonic deletions within your design?
- Can I include sample identification markers such as the Fluidigm SNP Trace sample ID panel?
- What species and targets do you design assays to?
- What is the minimum DNA input for assays using the Advanta NGS Library Prep Reagent Kit?
- What if I want singleplex assays?
- When is the use of preamplification recommended?
- Can I add other variants of interest that are not covered by the Advanta CFTR NGS Library Prep Assay?
- Your CF design only uses a portion of the assay inlets within the IFC. What am I supposed to do with the unused assay inlets?
- What is the process for redesigning assays?
- What are the maximum supported amplicon count and amplicon size?
- What is specific target amplification (STA)?
- What is provided with my order of SNP Type assays?
- How many Dynamic Array IFCs can I run with the provided assays?
- How do I determine the status of D3 assay design requests?
- What is the turnaround time for D3 assay design?
- Can SNP Type assays be wet-lab tested?
- What defines difficult genomic regions, and can D3 assay design be used for those regions?
- Does Fluidigm design to insertions or deletions (indels)?
- What types of SNPs can be targeted with D3 assay design?
- Can SNP Type assays be designed to targets with adjacent SNPs?
- How many bases upstream and downstream of target SNPs do I need to provide when submitting a D3 assay design request?
- Does Fluidigm design SNP Type assays for nonhuman species?
- How do I design and order SNP Type assays?
- What species and targets do you design Delta Gene gene expression assays to?
- Do you have any advice on primer design? View more
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Orders
- What is the cost of the product and what is the effective cost per sample?
- How do I order the product?
- How do I place orders?
- How do I place an order online?
- How can I see prices?
- What is available through online ordering?
- How do I set up an online ordering account?
- What payment options do you accept?
- What are the delivery options?
- Can Fluidigm sequester specific lots of reagents and/or consumables for my lab, and if so, what is the process for sequestering lots?
- How do I find my local distributor?
- How do I cancel, add or modify orders?
- How do I sign in to my account?
- How do I arrange credit terms?
- What currencies do you accept?
- How do I request a quote?
- What do I need to perform targeted DNA sequencing library preparation?
- What is provided with my order of Advanta NGS Library Prep assays?
- What information is provided in the customer informatics packet for the Advanta NGS Library Prep Reagent Kit?
- What is the process for redesigning assays?
- What information is provided in the genotyping Customer Informatics Packet?
- What is provided with my order of SNP Type assays?
- How many Dynamic Array IFCs can I run with the provided assays?
- How do I determine the status of D3 assay design requests?
- What is the turnaround time for D3 assay design?
- Can SNP Type assays be wet-lab tested?
- What defines difficult genomic regions, and can D3 assay design be used for those regions?
- Does Fluidigm design to insertions or deletions (indels)?
- What types of SNPs can be targeted with D3 assay design?
- Can SNP Type assays be designed to targets with adjacent SNPs?
- How many bases upstream and downstream of target SNPs do I need to provide when submitting a D3 assay design request?
- Does Fluidigm design SNP Type assays for nonhuman species?
- How do I design and order SNP Type assays?
- What information is provided in the Customer Informatics Packet?
- How do I supply my sales tax exemption?
- How do I disable my account?
- Can I use any email address for online ordering?
- How can I request a copy of my invoice?
- How can I track shipments?
- How do I view my online order history?
- I have a quote from my sales representative. Can I use that quote online?
- Can I order for multiple labs? View more
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Technical Service
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Consumables
- What is the shelf life for IFCs and Control Line Fluid?
- Can I use the LP 192.24 IFC on the AX Controller?
- Can I use my existing Access Array kit and assays on Juno with the Juno LP 192.24 IFC?
- How many integrated fluidic circuits (IFCs) can I run with the provided assays?
- Can the IFCs be centrifuged to get rid of bubbles?
- How much time can elapse between loading an IFC and thermal cycling?
- What is the difference between the Genotyping and Gene Expression IFCs?
- Can I reuse an IFC? Can I use part of an IFC, and then use the other part in another experiment?
- How quickly do I need to use the IFC after the foil pouch is opened?
- Why can’t I load the samples in the assay inlets and the assays in the sample inlets?
- What Fluidigm instruments are required for SNP genotyping?
- How should I store my genotyping assays?
- How long does it take to prime, load and run each IFC?
- What are the volumes for the chambers inside each of the IFCs? View more
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training
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Workflows
- Can you provide a summary of the assay workflow?
- How many samples can be processed per workday, and what is the total turnaround time from start to finish?
- Which sequencing systems are compatible with the Advanta NGS Library Prep Reagent Kit?
- What are the differences between Targeted DNA Seq Library Reagent Kits and Advanta NGS Library Prep Reagent Kits?
- Can I use the LP 192.24 IFC on the AX Controller?
- How many integrated fluidic circuits (IFCs) can I run with the provided assays?
- What level of manual input is required?
- What level of skill is required to perform the assay and how complex is the workflow?
- How much time can elapse between loading an IFC and thermal cycling?
- What if I don’t have exactly 48 or 96 samples or assays? Can I load water into my empty inlets?
- How many primer pairs can I include in a multiplex preamplification reaction?
- What is the minimum concentration of genomic DNA sample required for preamplification?
- Can I use any of the Fluidigm Sample Loading Reagents for my genotyping sample preparation?
- Can I use whole genome amplification (WGA) products as samples for genotyping?
- If my gDNA is less than the minimum required concentration for genotyping, what can I do?
- How many Dynamic Array IFCs can I run with the provided assays?
- What are some things I can try to improve SNP Type assay performance?
- What are the SNP Type Assay performance specifications?
- How should I store my SNP Type Assays?
- Can I use TaqMan Assays if the stock concentration is only 20X?
- Can I change the thermal cycling conditions for my genotyping reactions?
- How should I store my genotyping assays?
- What filters are included on the Biomark HD system?
- What is the maximum number of preamplification cycles I can do? View more
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Chemistry
- What is the minimum DNA input for assays using the Advanta NGS Library Prep Reagent Kit?
- What is provided with my order of Advanta NGS Library Prep assays?
- What if I want singleplex assays?
- How should I store my Advanta NGS Library Prep assay primers?
- Can I use my existing Access Array kit and assays on Juno with the Juno LP 192.24 IFC?
- How many integrated fluidic circuits (IFCs) can I run with the provided assays?
- What are SNP Type Assays?
- What are the maximum supported amplicon count and amplicon size?
- What PCR master mixes are supported for genotyping?
- How many primer pairs can I include in a multiplex preamplification reaction?
- What is the minimum concentration of genomic DNA sample required for preamplification?
- Can I use any of the Fluidigm Sample Loading Reagents for my genotyping sample preparation?
- Can I use whole genome amplification (WGA) products as samples for genotyping?
- If my gDNA is less than the minimum required concentration for genotyping, what can I do?
- Why can't I run real-time PCR with my SNP Type Assays?
- What fluorophores are used for SNP Type Genotyping? How do I know which fluorophore represents which allele?
- Can I use TaqMan Assays if the stock concentration is only 20X?
- What Fluidigm instruments are required for SNP genotyping?
- How do the SNP Type and TaqMan chemistries differ?
- What genotyping chemistries can be used on the Fluidigm platform?
- What filters are included on the Biomark HD system? View more
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Documents
We endeavor to think of everything when we develop a system, so we hope you’ll find all the details and explanations you need in the technical and support documents below. Simply click a title to open the document in your browser. To save a copy to your computer, click the Save button or press Ctrl + Shift + S.
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Data Sheets
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Protocols
- Advanta IO Gene Expression Assay Protocol
- Advanta NGS Preamplification Protocol
- Advanta CFTR NGS Library Preparation on the LP 48.48 IFC with Juno Protocol
- Advanta IO Gene Expression Assay Using the GE 24.192 IFC Protocol
- Advanta FFPE RNA Extraction Kit Protocol
- Advanta CFTR NGS Library Preparation on the LP 192.24 IFC with Juno Protocol
- Advanta NGS Library Preparation with Juno Protocol View more
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Quick References
- Juno Usage Quick Reference
- Genotyping with the Juno 96.96 IFC Using TaqMan Assays
- Advanta IO Gene Expression Assay Quick Reference
- Advanta FFPE RNA Extraction Kit Quick Reference
- Genotyping with the 96.96 Dynamic Array IFC Using the Advanta Sample ID Genotyping Panel Quick Reference
- Genotyping with the Juno 96.96 Genotyping IFC Using SNP Type Assays
- Targeted DNA Sequencing Library Preparation with the Juno LP—192.24 IFC Quick Reference
- Targeted DNA Sequencing Library Preparation with the LP—48.48 IFC on Juno Quick Reference
- Control Line Fluid Loading Procedure
- Control Line Fluid Loading Procedure
- Advanta IO Gene Expression Assay Using the GE 24.192 IFC Quick Reference
- Genomic DNA Preamplification Using the Advanta CFTR NGS Preamp Reagent Kit Quick Reference
- Advanta CFTR NGS Library Preparation on the LP 48.48 IFC with Juno Quick Reference
- Advanta CFTR NGS Library Preparation on the LP 192.24 IFC with Juno Quick Reference
- Advanta NGS Library Preparation on the LP 192.24 IFC with Juno Quick Reference
- Advanta NGS Library Preparation on the LP 48.48 IFC with Juno Quick Reference View more
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Release Notes
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Safety Data Sheets
- Fluidigm Custom Target Specific Assays Safety Data Sheet
- Advanta™ NGS Preamp Reagent Kit
- Advanta™ IO Gene Expression Reagent Kit—GE 24.192
- Advanta™ CFTR NGS Library Prep Assay—LP 192.24, 10 IFCs
- Advanta™ CFTR NGS Library Prep Assay—LP 48.48, 2 IFCs
- Control Line Fluid Safety Data Sheet
- Probe GT Master Mix, Juno™ SNP Type™ GT Master Mix Safety Data Sheet
- Juno™ GT Preamp Master Mix Safety Data Sheet
- Juno™ GT Flux Fluid Safety Data Sheet
- Targeted DNA Seq Library Reagent Kit v2 – 192.24 Safety Data Sheet
- Actuation Fluid Safety Data Sheet
- Juno SNP Type GT Master Mix View more
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Site Requirements
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Technical Notes
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User Guides
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Product Inserts
- Bulk Dilution Reagent, 25 μl Product Insert
- Juno 96.96 Genotyping Control Line Fluid
- Juno Genotyping Reagent Kit for 10 IFCs
- Juno Genotyping Kit for 10 IFCs
- Juno GT IFC and Control Line Fluid Kit
- Advanta IO Gene Expression Assay Panel A Assay Plate Product Insert
- Advanta IO Gene Expression Assay Reagent Kit—2 IFCs Product Insert
- Advanta IO Gene Expression Assay Panel B Assay Plate Product Insert
- Advanta IO Gene Expression Preamp Pool—Panel A Product Insert
- Advanta IO Gene Expression Control 1 Product Insert
- Advanta IO Gene Expression Preamp Pool—Panel B Product Insert
- Advanta NGS Library Prep Reagent Kit—LP 48.48, 2 IFCs Product Insert
- Advanta IO Gene Expression Reagent Kit—GE 24.192, 2 IFCs Product Insert
- Advanta IO Gene Expression Assay Reagent Kit—10 IFCs Product Insert
- Advanta FFPE RNA Extraction Kit Product Insert
- Advanta CFTR NGS Assay Pools Product Insert
- Advanta NGS Library Prep Reagent Kit—LP 192.24, 10 IFCs Product Insert
- Advanta NGS Library Prep Reagent Kits with TSP Sample Loading Reagent v2 Product Bulletin
- Advanta NGS Preamp Reagent Kit 960 Rxn Product Insert
- Advanta CFTR NGS Preamp Pool Product Insert
- Advanta NGS Library Prep Reagent Kit—LP 192.24, 2 IFCs Product Insert
- Advanta NGS Library Prep Reagent Bundle—LP 48.48, 10 IFCs Product Insert
- Juno LP 192.24 IFC with Accessories Product Insert
- Juno Control Line Fluid 192.24 Product Insert
- 192.24 Targeted DNA Seq Library Assay Loading Reagent Product Insert
- Targeted DNA Seq Library Adapter Mix Product Insert
- Targeted DNA Seq Library Adapter Set Product Insert
- 192.24 Targeted DNA Seq Library Barcode Plates Product Insert
- LP—48.48 Control Line Fluid Product Insert
- LP—48.48 IFC with Accessories Product Insert
- 192.24 Targeted DNA Seq Library Harvest Reagent Product Insert
- Advanta NGS Preamp Reagent Kit—96 Rxn Product Insert
- Targeted DNA Seq Library Reagent Kit—48.48, 2 IFCs Product Insert
- Targeted DNA Seq Library Reagent Bundle—48.48, 10 IFCs Product Insert
- Targeted DNA Seq Library Reagent Module—48.48, 10 IFCs Product Insert
- SNP Type Assays and SNP Trace Panel Product Insert
- Juno SNP Type Genotyping Kit
- Juno SNP Type Genotyping Kit for 10 IFCs
- 192.24 Targeted DNA Seq Library Reagent Kit
- Targeted DNA Seq Library Reagent Kit—192.24, 2 IFCs
- LP—48.48 Barrier Tape
- Juno LP 192.24 Barrier Tape
- Juno Accessories Kit
- Juno LP Upgrade Kit View more
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Training & Services
At Fluidigm, we’re proud to create biotech tools that enable our customers to seek truth in life sciences. We’re dedicated to working with you to empower you however we can. That’s why we’ve developed a range of training programs to help you make the most of your Juno system.
Fluidigm is dedicated to providing industry-leading technology and tools that enable you to achieve more in life sciences. And to help you stay ahead, we stand behind our work, offering exceptional service plans and options to keep your Juno instrument in top condition.
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Consumables
Transform your sample-prep workflow with innovative IFCs designed specifically to leverage the simplicity and power of Juno, as well as SNP Type assays, SNP Trace panels and more.
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Contact Support
CyTOF & Helios
T: 650 266 6100
Toll Free: 866 358 4354 in the US
Press 5 for CyTOF inquiries when operator is logged offReach out to our global support team here.