Explore tutorials and publications, browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
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Publications
Introduced in 2006 and updated in 2011, Biomark™ HD stands alone in the world of analytical instrumentation as a multiapplication platform without compromise, providing high-quality results for every experimental approach. Using a wide variety of sample types and chemistry choices to accommodate the most genomic applications, Biomark HD lets users run entire projects from research to validation to routine screening on a single system with the flexibility and consistency necessary for today’s biological research.
Browse our catalog of related publications to learn how Biomark empowers researchers across a range of genomic fields.
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FAQs
We’ve answered some of the most relevant recurring questions about Biomark systems to ensure that you have the information you need to succeed in your research. For additional information, check out the tutorials and technical documents or inquire about one of our training programs.
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Applications
- How much tissue should I start with?
- My extracted RNA yield is lower than expected. What could be causing that?
- What kind of magnetic separator do you recommend?
- What do you recommend as FFPE RNA reference material for control?
- What is DV200?
- What is the final elution volume of the Advanta FFPE RNA Extraction Kit Protocol?
- How is the concentration per panel calculated in Digital PCR?
- What is the difference between Hits and Estimated Targets?
- What is the recommended input concentration for Digital PCR IFCs?
- How long can I store extracted material?
- Can I use my own qPCR master mix?
- What methods are supported by the Juno instrument?
- What are the part numbers for ordering the Advanta IO Gene Expression Assay?
- Is preamplification required in the Advanta IO Gene Expression Assay?
- What is the Advanta IO Gene Expression Assay?
- What is the Advanta IO Gene Expression Control 1 and how is it used?
- How should I perform data analysis for the Advanta IO Gene Expression Assay?
- Which software version should I use for the Advanta IO Gene Expression Assay?
- What additional materials are needed to use the Advanta IO Gene Expression Assay kit?
- Can I automate use of the Advanta IO Gene Expression Assay with my liquid handler?
- What reagents are required to run the Advanta IO Gene Expression Assay?
- What equipment is required to use the Advanta IO Gene Expression Assay?
- Which IFC is used for the Advanta IO Gene Expression Assay?
- I already have IFCs. Can I just buy the assays for the Advanta IO Gene Expression Assay?
- Do I have to purchase both panels of the Advanta IO Gene Expression Assay?
- Is it possible to add assays to the Advanta IO Gene Expression Assay?
- What chemistry is used for the Advanta IO Gene Expression Assay?
- What genes are covered by the Advanta IO Gene Expression Assay?
- How many genes are in the Advanta IO Gene Expression Assay?
- What analysis settings should be used for the Advanta IO Gene Expression Assay?
- Why do I need to prepare fresh 80% ethanol?
- Can I still use RNA integrity number (RIN)?
- How can I assess RNA quantity and integrity?
- What types of samples could be used with the Advanta FFPE RNA Extraction Kit?
- What level of manual input is required for the Advanta IO Gene Expression Assay?
- What is the workflow of the Advanta IO Gene Expression Assay?
- Is the Advanta IO Gene Expression Assay a single-cell assay?
- What sample types are supported for the Advanta IO Gene Expression Assay?
- What is the sample RNA input amount for the Advanta IO Gene Expression Assay?
- What are the performance characteristics of the Advanta IO Gene Expression Assay?
- Which genes should I use as reference with the Advanta IO Gene Expression Assay to normalize expression?
- What level of skill is required to perform the Advanta IO Gene Expression Assay (workflow complexity)?
- How many assays can be included in a single Advanta IO Gene Expression Assay run?
- How many runs can be accomplished in a single day with the Advanta IO Gene Expression Assay?
- How many samples can be processed in a single run of the Advanta IO Gene Expression Assay?
- Can the 24.192 GE IFC be used for genotyping?
- How do Ct values on the 24.192 GE IFC differ from values obtained on the Biomark 96.96 GE IFC?
- Is the new 24.192 Dynamic Array IFC compatible with Delta Gene chemistry?
- Is the 24.192 GE IFC compatible with Fast TaqMan chemistry?
- What gene expression chemistries are compatible with the 24.192 GE IFC?
- What qPCR master mixes are recommended for the 24.192 GE IFC?
- What is the difference between the Biomark 24.192 Complete Bundle and the 24.192 GE IFC & Reagent Kit?
- Can I use Biomark HD to screen samples from mRNA seq harvest?
- Can I prepare a master mix for steps that require mixing multiple reagents?
- For which applications can I use extracted RNA?
- Can I purchase hexadecane from any vendor?
- Can I purchase ethanol from any vendor?
- Can I use the kit to extract DNA from FFPE samples? View more
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Software
- How is the concentration per panel calculated in Digital PCR?
- What is the difference between Hits and Estimated Targets?
- How many biological replicates (cells) should be used for single-cell analysis on the C1 system?
- What types of data can be analyzed for gene expression by the Singular Analysis Toolset?
- Can I run Fluidigm data analysis software on a Mac?
- What types of analysis software do people usually use after your platform?
- Can I combine multiple IFC runs in the Real-Time PCR Analysis software?
- Can the Tm threshold value in the melting curve be used for eliminating any nonspecific amplification or primer dimers?
- What is principal component analysis (PCA)?
- What is the basic method for generating hierarchical clusters?
- I detected thousands of genes in my RNA sequencing data. Why does the Singular Analysis Toolset display only a limited number of them?
- Can I do DNA methylation studies?
- What are the software and firmware requirements for Flex Six IFC?
- What is the difference between SNP Type Normalization and no template control (NTC) normalization?
- What is the difference between NO CALL and an Invalid call in the Fluidigm Genotyping Analysis software?
- What can I do to help improve call rates?
- How does the Fluidigm SNP Genotyping Analysis software determine the genotype call for a sample?
- When should I use the All SNP Scatter Plot View option in the SNP Genotyping Analysis software?
- How do I set up ROX as a probe?
- Why is there a 10 Ct shift from tube-based qPCR to the Biomark system?
- Do you provide any software for single-cell gene expression analysis?
- What is the linear derivative baseline correction and how is it calculated?
- What is the Quality Threshold score, how is it calculated and why does it fail my data?
- How does the Biomark Data Analysis software calculate ddCt?
- How do I interpret multiple Tm peaks for an assay?
- What is the software requirement for running Delta Gene assays? View more
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Design
- What is specific target amplification (STA)?
- What is provided with my order of SNP Type assays?
- How many Dynamic Array IFCs can I run with the provided assays?
- How do I determine the status of D3 assay design requests?
- What is the turnaround time for D3 assay design?
- Can SNP Type assays be wet-lab tested?
- What defines difficult genomic regions, and can D3 assay design be used for those regions?
- Does Fluidigm design to insertions or deletions (indels)?
- What types of SNPs can be targeted with D3 assay design?
- Can SNP Type assays be designed to targets with adjacent SNPs?
- How many bases upstream and downstream of target SNPs do I need to provide when submitting a D3 assay design request?
- Does Fluidigm design SNP Type assays for nonhuman species?
- How do I design and order SNP Type assays?
- What does a no call Delta Gene assay test result mean?
- What is the turnaround time for delivery of Delta Gene assays?
- What are the “Biologically Grouped Assays” on the Delta Gene order form?
- What species and targets do you design Delta Gene gene expression assays to?
- How are Delta Gene assays wet-lab tested, and how do I interpret the dCt performance report provided with wet-lab tested assays?
- What are Delta Gene assays?
- Do you have any advice on primer design? View more
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Orders
- How can I see prices?
- What is included in the Biomark HD Premium Qualification Service Plan?
- What is included in a Preventive Maintenance (PM) visit?
- What are the key benefits of the Biomark HD Standard Service Plan?
- How do I place orders?
- How do I place an order online?
- What is available through online ordering?
- How do I set up an online ordering account?
- What payment options do you accept?
- What are the delivery options?
- What is included in the IFC Controller Premium Certification Service Plan?
- How do I find my local distributor?
- How do I cancel, add or modify orders?
- How do I sign in to my account?
- How do I arrange credit terms?
- What currencies do you accept?
- How do I request a quote?
- What is the difference between the Biomark 24.192 Complete Bundle and the 24.192 GE IFC & Reagent Kit?
- Can I purchase the 24.192 GE IFC independently from the reagent kit?
- What information is provided in the genotyping Customer Informatics Packet?
- What is provided with my order of SNP Type assays?
- How many Dynamic Array IFCs can I run with the provided assays?
- How do I determine the status of D3 assay design requests?
- What is the turnaround time for D3 assay design?
- Can SNP Type assays be wet-lab tested?
- What defines difficult genomic regions, and can D3 assay design be used for those regions?
- Does Fluidigm design to insertions or deletions (indels)?
- What types of SNPs can be targeted with D3 assay design?
- Can SNP Type assays be designed to targets with adjacent SNPs?
- How many bases upstream and downstream of target SNPs do I need to provide when submitting a D3 assay design request?
- Does Fluidigm design SNP Type assays for nonhuman species?
- How do I design and order SNP Type assays?
- How do I reorder Delta Gene assays?
- How do I order Delta Gene assays?
- What does a no call Delta Gene assay test result mean?
- What is the turnaround time for delivery of Delta Gene assays?
- What are the “Biologically Grouped Assays” on the Delta Gene order form?
- What components are in the DNA Binding Dye Sample & Assay Loading kits?
- What other components are required to run Delta Gene assays?
- What information is provided in the Customer Informatics Packet?
- What is provided with my order of Delta Gene assays?
- What is included in the IFC Controller and FC1 Cycler Depot Repair Service Plans?
- What are the key benefits of the IFC Controller and FC1 Cycler Depot Repair Service Plans?
- How do I supply my sales tax exemption?
- How do I disable my account?
- Can I use any email address for online ordering?
- How can I request a copy of my invoice?
- How can I track shipments?
- How do I view my online order history?
- I have a quote from my sales representative. Can I use that quote online?
- Can I order for multiple labs? View more
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Technical Service
- What is included in the Biomark HD Premium Qualification Service Plan?
- What is included in a Preventive Maintenance (PM) visit?
- What are the key benefits of the Biomark HD Standard Service Plan?
- What is included in the IFC Controller Premium Certification Service Plan?
- What are the key benefits of the IFC Controller Premium Certification Service Plan?
- What is included in the IFC Controller and FC1 Cycler Depot Repair Service Plans?
- What are the key benefits of the IFC Controller and FC1 Cycler Depot Repair Service Plans?
- How do I subscribe to the Fluidigm newsletter? View more
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Consumables
- How much tissue should I start with?
- What is the shelf life for IFCs and Control Line Fluid?
- My extracted RNA yield is lower than expected. What could be causing that?
- What kind of magnetic separator do you recommend?
- What do you recommend as FFPE RNA reference material for control?
- What is DV200?
- What is the final elution volume of the Advanta FFPE RNA Extraction Kit Protocol?
- How long can I store extracted material?
- How should I store my SNP Type genotyping assays?
- Can I use my own qPCR master mix?
- What are the part numbers for ordering the Advanta IO Gene Expression Assay?
- Is preamplification required in the Advanta IO Gene Expression Assay?
- What is the Advanta IO Gene Expression Assay?
- What is the Advanta IO Gene Expression Control 1 and how is it used?
- How should I perform data analysis for the Advanta IO Gene Expression Assay?
- Which software version should I use for the Advanta IO Gene Expression Assay?
- What additional materials are needed to use the Advanta IO Gene Expression Assay kit?
- Can I automate use of the Advanta IO Gene Expression Assay with my liquid handler?
- What reagents are required to run the Advanta IO Gene Expression Assay?
- What equipment is required to use the Advanta IO Gene Expression Assay?
- Which IFC is used for the Advanta IO Gene Expression Assay?
- I already have IFCs. Can I just buy the assays for the Advanta IO Gene Expression Assay?
- Do I have to purchase both panels of the Advanta IO Gene Expression Assay?
- Is it possible to add assays to the Advanta IO Gene Expression Assay?
- What chemistry is used for the Advanta IO Gene Expression Assay?
- What genes are covered by the Advanta IO Gene Expression Assay?
- How many genes are in the Advanta IO Gene Expression Assay?
- What analysis settings should be used for the Advanta IO Gene Expression Assay?
- Why do I need to prepare fresh 80% ethanol?
- Can I still use RNA integrity number (RIN)?
- How can I assess RNA quantity and integrity?
- How many samples could be processed with the Advanta FFPE RNA Extraction Kit?
- What types of samples could be used with the Advanta FFPE RNA Extraction Kit?
- What level of manual input is required for the Advanta IO Gene Expression Assay?
- What is the workflow of the Advanta IO Gene Expression Assay?
- Is the Advanta IO Gene Expression Assay a single-cell assay?
- What sample types are supported for the Advanta IO Gene Expression Assay?
- What is the sample RNA input amount for the Advanta IO Gene Expression Assay?
- What are the performance characteristics of the Advanta IO Gene Expression Assay?
- Which genes should I use as reference with the Advanta IO Gene Expression Assay to normalize expression?
- What level of skill is required to perform the Advanta IO Gene Expression Assay (workflow complexity)?
- How many assays can be included in a single Advanta IO Gene Expression Assay run?
- How many runs can be accomplished in a single day with the Advanta IO Gene Expression Assay?
- How many samples can be processed in a single run of the Advanta IO Gene Expression Assay?
- Can the 24.192 GE IFC be used for genotyping?
- How do Ct values on the 24.192 GE IFC differ from values obtained on the Biomark 96.96 GE IFC?
- Is the new 24.192 Dynamic Array IFC compatible with Delta Gene chemistry?
- Is the 24.192 GE IFC compatible with Fast TaqMan chemistry?
- What gene expression chemistries are compatible with the 24.192 GE IFC?
- What qPCR master mixes are recommended for the 24.192 GE IFC?
- What is the difference between the Biomark 24.192 Complete Bundle and the 24.192 GE IFC & Reagent Kit?
- Can I purchase the 24.192 GE IFC independently from the reagent kit?
- Can the IFCs be centrifuged to get rid of bubbles?
- If I have multiple IFC runs, can I prepare my sample mix and assay mix the day before? A week before?
- How should I store Flex Six IFCs between use?
- What master mixes can I use with TaqMan gene expression assays on Fluidigm IFCs?
- What are the software and firmware requirements for Flex Six IFC?
- How much time can elapse between loading an IFC and thermal cycling?
- What is the difference between the Genotyping and Gene Expression IFCs?
- Can I reuse an IFC? Can I use part of an IFC, and then use the other part in another experiment?
- How quickly do I need to use the IFC after the foil pouch is opened?
- Why can’t I load the samples in the assay inlets and the assays in the sample inlets?
- What Fluidigm instruments are required for SNP genotyping?
- Am I required to load a no-template control (NTC) in sample inlet 22 of the 48.48 Dynamic Array IFC?
- Can I use master mixes other than the ones listed in the Fluidigm protocols for gene expression using Delta Gene assays?
- Which reverse transcription kits do you recommend?
- How do I reorder Delta Gene assays?
- How do I order Delta Gene assays?
- What components are in the DNA Binding Dye Sample & Assay Loading kits?
- What other components are required to run Delta Gene assays?
- Can SYBR Green be used?
- Can I use Biomark HD to screen samples from mRNA seq harvest?
- Can I prepare a master mix for steps that require mixing multiple reagents?
- For which applications can I use extracted RNA?
- Can I purchase hexadecane from any vendor?
- Can I purchase ethanol from any vendor?
- Can I use the kit to extract DNA from FFPE samples?
- How long does it take to prime, load and run each IFC?
- What are the volumes for the chambers inside each of the IFCs? View more
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Workflows
- How much tissue should I start with?
- My extracted RNA yield is lower than expected. What could be causing that?
- What kind of magnetic separator do you recommend?
- What do you recommend as FFPE RNA reference material for control?
- What is DV200?
- What is the final elution volume of the Advanta FFPE RNA Extraction Kit Protocol?
- How long can I store extracted material?
- How should I store my SNP Type genotyping assays?
- Can I use my own qPCR master mix?
- How many biological replicates (cells) should be used for single-cell analysis on the C1 system?
- What are the part numbers for ordering the Advanta IO Gene Expression Assay?
- Is preamplification required in the Advanta IO Gene Expression Assay?
- What is the Advanta IO Gene Expression Assay?
- What is the Advanta IO Gene Expression Control 1 and how is it used?
- How should I perform data analysis for the Advanta IO Gene Expression Assay?
- Which software version should I use for the Advanta IO Gene Expression Assay?
- What additional materials are needed to use the Advanta IO Gene Expression Assay kit?
- Can I automate use of the Advanta IO Gene Expression Assay with my liquid handler?
- What reagents are required to run the Advanta IO Gene Expression Assay?
- What equipment is required to use the Advanta IO Gene Expression Assay?
- Which IFC is used for the Advanta IO Gene Expression Assay?
- I already have IFCs. Can I just buy the assays for the Advanta IO Gene Expression Assay?
- Do I have to purchase both panels of the Advanta IO Gene Expression Assay?
- Is it possible to add assays to the Advanta IO Gene Expression Assay?
- What chemistry is used for the Advanta IO Gene Expression Assay?
- What genes are covered by the Advanta IO Gene Expression Assay?
- How many genes are in the Advanta IO Gene Expression Assay?
- What analysis settings should be used for the Advanta IO Gene Expression Assay?
- Why do I need to prepare fresh 80% ethanol?
- Can I still use RNA integrity number (RIN)?
- How can I assess RNA quantity and integrity?
- How many samples could be processed with the Advanta FFPE RNA Extraction Kit?
- What types of samples could be used with the Advanta FFPE RNA Extraction Kit?
- What level of manual input is required for the Advanta IO Gene Expression Assay?
- What is the workflow of the Advanta IO Gene Expression Assay?
- Is the Advanta IO Gene Expression Assay a single-cell assay?
- What sample types are supported for the Advanta IO Gene Expression Assay?
- What is the sample RNA input amount for the Advanta IO Gene Expression Assay?
- What are the performance characteristics of the Advanta IO Gene Expression Assay?
- Which genes should I use as reference with the Advanta IO Gene Expression Assay to normalize expression?
- What level of skill is required to perform the Advanta IO Gene Expression Assay (workflow complexity)?
- How many assays can be included in a single Advanta IO Gene Expression Assay run?
- How many runs can be accomplished in a single day with the Advanta IO Gene Expression Assay?
- How many samples can be processed in a single run of the Advanta IO Gene Expression Assay?
- Can the 24.192 GE IFC be used for genotyping?
- How do Ct values on the 24.192 GE IFC differ from values obtained on the Biomark 96.96 GE IFC?
- Is the new 24.192 Dynamic Array IFC compatible with Delta Gene chemistry?
- Is the 24.192 GE IFC compatible with Fast TaqMan chemistry?
- What gene expression chemistries are compatible with the 24.192 GE IFC?
- What qPCR master mixes are recommended for the 24.192 GE IFC?
- What is the difference between the Biomark 24.192 Complete Bundle and the 24.192 GE IFC & Reagent Kit?
- If I have multiple IFC runs, can I prepare my sample mix and assay mix the day before? A week before?
- Can I combine multiple IFC runs in the Real-Time PCR Analysis software?
- How do I obtain single cells for gene expression analysis, and how are single cells collected?
- Does Fluidigm have any optimization and validation guidelines for single-cell gene expression assays?
- How much time can elapse between loading an IFC and thermal cycling?
- What if I don’t have exactly 48 or 96 samples or assays? Can I load water into my empty inlets?
- How should I dilute preamplification products after amplification?
- Will preamplification introduce amplification bias?
- How many primer pairs can I include in a multiplex preamplification reaction?
- What is the minimum concentration of genomic DNA sample required for preamplification?
- When should I perform preamplification prior to SNP genotyping on the Fluidigm platform?
- Can I use any of the Fluidigm Sample Loading Reagents for my genotyping sample preparation?
- Can I use whole genome amplification (WGA) products as samples for genotyping?
- If my gDNA is less than the minimum required concentration for genotyping, what can I do?
- How many Dynamic Array IFCs can I run with the provided assays?
- What are some things I can try to improve SNP Type assay performance?
- What are the SNP Type Assay performance specifications?
- How should I store my SNP Type Assays?
- Can I use TaqMan Assays if the stock concentration is only 20X?
- Can I change the thermal cycling conditions for my genotyping reactions?
- How do I set up ROX as a probe?
- Am I required to load a no-template control (NTC) in sample inlet 22 of the 48.48 Dynamic Array IFC?
- Do I have to dilute my reaction products 5X after preamplification?
- What filters are included on the Biomark HD system?
- Why is the Exo I treatment after preamplification needed only for EvaGreen?
- What is the maximum number of preamplification cycles I can do?
- Do I need preamplification for gene expression? Why?
- Can I use Biomark HD to screen samples from mRNA seq harvest?
- Can I prepare a master mix for steps that require mixing multiple reagents?
- For which applications can I use extracted RNA?
- Can I purchase hexadecane from any vendor?
- Can I purchase ethanol from any vendor?
- Can I use the kit to extract DNA from FFPE samples?
- If I’m using small numbers of cells, should I use the Fluidigm single-cell protocol or the regular Fluidigm protocol for bulk gene expression? View more
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Chemistry
- How much tissue should I start with?
- My extracted RNA yield is lower than expected. What could be causing that?
- What kind of magnetic separator do you recommend?
- What do you recommend as FFPE RNA reference material for control?
- What is DV200?
- What is the final elution volume of the Advanta FFPE RNA Extraction Kit Protocol?
- What is the recommended input concentration for Digital PCR IFCs?
- Can I run EvaGreen chemistry on the Digital PCR IFCs?
- How long can I store extracted material?
- Can I use my own qPCR master mix?
- What are SNP Type Assays?
- What are the part numbers for ordering the Advanta IO Gene Expression Assay?
- Is preamplification required in the Advanta IO Gene Expression Assay?
- What is the Advanta IO Gene Expression Assay?
- What is the Advanta IO Gene Expression Control 1 and how is it used?
- How should I perform data analysis for the Advanta IO Gene Expression Assay?
- Which software version should I use for the Advanta IO Gene Expression Assay?
- What additional materials are needed to use the Advanta IO Gene Expression Assay kit?
- Can I automate use of the Advanta IO Gene Expression Assay with my liquid handler?
- What reagents are required to run the Advanta IO Gene Expression Assay?
- What equipment is required to use the Advanta IO Gene Expression Assay?
- Which IFC is used for the Advanta IO Gene Expression Assay?
- I already have IFCs. Can I just buy the assays for the Advanta IO Gene Expression Assay?
- Do I have to purchase both panels of the Advanta IO Gene Expression Assay?
- Is it possible to add assays to the Advanta IO Gene Expression Assay?
- What chemistry is used for the Advanta IO Gene Expression Assay?
- What genes are covered by the Advanta IO Gene Expression Assay?
- How many genes are in the Advanta IO Gene Expression Assay?
- What analysis settings should be used for the Advanta IO Gene Expression Assay?
- Why do I need to prepare fresh 80% ethanol?
- Can I still use RNA integrity number (RIN)?
- How can I assess RNA quantity and integrity?
- What types of samples could be used with the Advanta FFPE RNA Extraction Kit?
- What level of manual input is required for the Advanta IO Gene Expression Assay?
- What is the workflow of the Advanta IO Gene Expression Assay?
- Is the Advanta IO Gene Expression Assay a single-cell assay?
- What sample types are supported for the Advanta IO Gene Expression Assay?
- What is the sample RNA input amount for the Advanta IO Gene Expression Assay?
- What are the performance characteristics of the Advanta IO Gene Expression Assay?
- Which genes should I use as reference with the Advanta IO Gene Expression Assay to normalize expression?
- What level of skill is required to perform the Advanta IO Gene Expression Assay (workflow complexity)?
- How many assays can be included in a single Advanta IO Gene Expression Assay run?
- How many runs can be accomplished in a single day with the Advanta IO Gene Expression Assay?
- How many samples can be processed in a single run of the Advanta IO Gene Expression Assay?
- Can the 24.192 GE IFC be used for genotyping?
- Is the new 24.192 Dynamic Array IFC compatible with Delta Gene chemistry?
- Is the 24.192 GE IFC compatible with Fast TaqMan chemistry?
- What gene expression chemistries are compatible with the 24.192 GE IFC?
- What qPCR master mixes are recommended for the 24.192 GE IFC?
- What master mixes can I use with TaqMan gene expression assays on Fluidigm IFCs?
- What PCR master mixes are supported for genotyping?
- What is the detection limit of target copy number per chamber for qPCR on the Biomark HD system? Is there a Ct cutoff?
- How should I dilute preamplification products after amplification?
- Will preamplification introduce amplification bias?
- How many primer pairs can I include in a multiplex preamplification reaction?
- What is the minimum concentration of genomic DNA sample required for preamplification?
- Can I use any of the Fluidigm Sample Loading Reagents for my genotyping sample preparation?
- Can I use whole genome amplification (WGA) products as samples for genotyping?
- If my gDNA is less than the minimum required concentration for genotyping, what can I do?
- What is the minimum genomic DNA (gDNA) concentration required for genotyping on the Biomark, Biomark HD or EP1 system? Why?
- Why can't I run real-time PCR with my SNP Type Assays?
- What fluorophores are used for SNP Type Genotyping? How do I know which fluorophore represents which allele?
- Can I use TaqMan Assays if the stock concentration is only 20X?
- What Fluidigm instruments are required for SNP genotyping?
- How do the SNP Type and TaqMan chemistries differ?
- What genotyping chemistries can be used on the Fluidigm platform?
- Can I use master mixes other than the ones listed in the Fluidigm protocols for gene expression using Delta Gene assays?
- Do I have to dilute my reaction products 5X after preamplification?
- How much RNA is needed for the reverse transcriptase and preamplification steps of RT-PCR?
- Which reverse transcription kits do you recommend?
- What is the difference between EvaGreen and TaqMan?
- How do I interpret multiple Tm peaks for an assay?
- What other components are required to run Delta Gene assays?
- What filters are included on the Biomark HD system?
- Can SYBR Green be used?
- Can I prepare a master mix for steps that require mixing multiple reagents?
- For which applications can I use extracted RNA?
- Can I purchase hexadecane from any vendor?
- Can I purchase ethanol from any vendor?
- Can I use the kit to extract DNA from FFPE samples? View more
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Documents
We endeavor to think of everything when we develop a system, so we hope you’ll find all the details and explanations you need in the technical and support documents below. Simply click a title to open the document in your browser.
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Brochures
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Posters
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Protocols
- Pathogen Detection Using the Advanta RT-PCR Kit (FLDM-00191 Rev 02 PROTOCOL)
- Protocol, Real-Time PCR for Viral Detection
- Advanta IO GE Assays Protocol
- Advanta IO Gene Expression Assay Protocol
- Advanta IO Gene Expression Assay Using the GE 24.192 IFC Protocol
- Advanta FFPE RNA Extraction Kit Protocol
- Gene Expression Analysis with the GE 24.192 IFC Using TaqMan Assays Protocol
- 4-Primer Amplicon Tagging on Biomark HD Protocol View more
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Quick References
- Pathogen Detection Using the Advanta RT-PCR Kit(FLDM-00192 Rev 02)
- Gene Expression Specific Target Amplification
- Real-Time PCR for Viral RNA Detection Quick Reference
- Genotyping with the 96.96 Dynamic Array IFC Using the Advanta Sample ID Genotyping Panel Quick Reference
- Advanta IO Gene Expression Assay Quick Reference
- Advanta FFPE RNA Extraction Kit (FAQs) 101-7071
- Advanta FFPE RNA Extraction Kit Quick Reference
- Gene Expression with the 96.96 IFC Using Delta Gene Assays on Preamplified Samples
- Gene Expression with the 48.48 IFC Using Delta Gene Assays on Preamplified Samples
- Preamplification of cDNA for Gene Expression with Delta Gene Assays
- Updating the Biomark/EP1 Software Quick Reference
- 48.48 GT TaqMan Standard Workflow Quick Reference
- 12.765 Digital PCR Workflow Quick Reference
- Stand-Alone Thermal Cycler
- qdPCR 37K Digital PCR Workflow Quick Reference
- IFC Controller Usage
- IFC Controller Setup
- High-Precision 96.96 GT Workflow
- GE PreAmp Master Mix (TaqMan)
- FR48.48 GT TaqMan Workflow Quick Reference
- FR48.48 GT SNP Type Workflow
- FR48.48 Cleaning Workflow (Wash and Bake)
- Flex Six GT TaqMan Fast/Standard Workflow
- Flex Six GT SNP Type Fast/Standard Workflow
- Flex Six GE TaqMan Fast/Standard Workflow
- Flex Six GE Delta Gene Fast/Standard Workflow
- FC1 Cycler Setup
- cDNA Prep Reverse Transcription Master Mix
- 96.96 GE TaqMan Standard Workflow
- FC1 Cycler Usage
- Gene Expression Analysis with the GE 24.192 IFC Using TaqMan Assays
- Advanta IO Gene Expression Assay Using the GE 24.192 IFC Quick Reference
- 48.48 GT SNP Type Workflow
- 48.48 GT TaqMan Fast Workflow Quick Reference
- 96.96 SNP Type Assay and SNP Trace Panel Workflow Quick Reference
- 96.96 GT TaqMan Fast Workflow
- 96.96 GE TaqMan Fast Workflow
- 192.24 GT SNP Type Workflow Quick Reference
- 48.770 Digital PCR Workflow Quick Reference
- 192.24 GE TaqMan Fast Workflow
- 96.96 GT TaqMan Standard Workflow
- 192.24 GT TaqMan Fast Workflow Quick Reference
- 192.24 GE Delta Gene Fast/Standard Workflow
- 192.24 GE TaqMan Standard Workflow
- 48.48 GE TaqMan Standard Workflow Quick Reference
- 48.48 GE TaqMan Fast Workflow Quick Reference View more
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Release Notes
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Safety Data Sheets
- Advanta™ RNA-Seq Unique Dual Index, Modules 3A and 3B
- Advanta™ RNA-Seq Reagent Kit, Module 2 (1 of 3; Salt Buffers) Safety Data Sheet
- Fluidigm Custom Target Specific Assays Safety Data Sheet
- Advanta™ RNA-Seq Reagent Kit, Module 4 Safety Data Sheet
- Advanta™ RNA-Seq Reagent Kit, Module 1 Safety Data Sheet
- PCR Water Safety Data Sheet
- Advanta™ RNA-Seq Reagent Kit, Module 2 (3 of 3; Surfactant Buffers) Safety Data Sheet
- Advanta™ RNA-Seq Reagent Kit, Module 2 (2 of 3; Enzymes) Safety Data Sheet
- Control Line Fluid Safety Data Sheet
- 5X TSP RT Buffer
- TSP RNase Inhibitor
- TSP Reverse Transcriptase
- 96.96 GT PCR Dilution Reagent
- 20X GT Sample Loading Reagent Safety Data Sheet
- Advanta™ FFPE RNA Extraction Kit, 50 reactions
- Biomark™ 24.192 GE Reagent Kit—10 IFCs
- Biomark™ 24.192 GE Reagent Kit—2 IFCs
- 20X GE Sample Loading Reagent
- PreAmp Master Mix Safety Data Sheet
- Advanta Immuno-Oncology Gene Expression Reagent Kit - 2 IFC Safety Data Sheet
- Actuation Fluid Safety Data Sheet
- 20X Fast GT Sample Loading Reagent View more
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Site Requirements
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Technical Notes
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User Guides
- Biomark HD Data Collection User Guide
- Real-Time PCR Analysis User Guide
- SNP Genotyping Analysis User Guide
- Melting Curve Analysis (MCA) User Guide
- SNP Trace Panel User Guide
- Biomark/EP1 Data Collection User Guide
- IFC Controller RX
- D3 Assay Design User Guide
- IFC Controller MX-HX
- IFC Controller WX
- Genomics Instruments Safety Guide
- Digital PCR Analysis User Guide
- FC1 Cycler View more
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Product Inserts
- Product Insert, Advanta RT-PCR Kit—192.24, 2 IFCs
- Product Insert, Advanta RT-PCR Kit—192.24, 10 IFCs
- Product Insert, GE 192.24 Dynamic Array 4X Reagent Kit—10 IFCs
- Product Insert, Advanta RNA Extraction Kit
- Product Insert, 4X Assay Loading Reagent—5 Tubes
- 96.96 DNA Binding Dye Sample/Loading Kit— 10 IFCs
- GE 48.48 Dynamic Array Sample & Assay Loading Reagent Kit—10 IFCs
- 48.48 DNA Binding Dye Sample/Loading Kit— 10 IFCs
- 2X Assay Loading Reagent—5 Tubes
- 20X GE Sample Loading Reagent—5 Tubes
- 20X DNA Binding Dye—5 Tubes
- 20X SNP Type Sample Loading Reagent—5 Tubes
- 60X SNP Type Reagent—5 Tubes
- Flex Six SNP Type Genotyping Reagent Kit—5 IFCs
- Flex Six Gene Expression Kit—5 IFCs
- Preamp and Reverse Transcription Master Mix— 5 Tubes
- Reverse Transcription Master Mix—5 Tubes
- Reverse Transcription Master Mix—1 Tube
- 192.24 GE Dynamic Array Reagent Kit—10 IFCs
- 192.24 GE IFC & Reagent Kit—10 IFCs
- Preamp Master Mix—5 Tubes
- Preamp Master Mix—1 Tube
- GE 96.96 Dynamic Array DNA Binding Dye Sample & Assay Loading Reagent Kit—10 IFCs
- GE 96.96 Dynamic Array DNA Binding Dye Sample & Assay Loading Reagent Kit with Control Line Fluid—10 IFCs
- GE 48.48 Dynamic Array DNA Binding Dye Sample & Assay Loading Reagent Kit—10 IFCs
- Advanta Immuno-Oncology GE Panel-A Product Insert
- Advanta IO Gene Expression Assay Panel A Assay Plate Product Insert
- Advanta IO Gene Expression Assay Reagent Kit—2 IFCs Product Insert
- Advanta IO Gene Expression Assay Panel B Assay Plate Product Insert
- Advanta IO Gene Expression Preamp Pool—Panel A Product Insert
- Advanta Immuno-Oncology GE Panel-B Product Insert
- Advanta IO Gene Expression Control 1 Product Insert
- Advanta IO Gene Expression Preamp Pool—Panel B Product Insert
- Advanta IO Gene Expression Reagent Kit—GE 24.192, 2 IFCs Product Insert
- Advanta IO Gene Expression Assay Reagent Kit—10 IFCs Product Insert
- Advanta FFPE RNA Extraction Kit Product Insert
- Biomark 24.192 GE Dynamic Array Reagent Kit— 2 IFCs Product Insert
- Preamp and Reverse Transcription Master Mix— 4 Tubes Product Insert
- Preamp and Reverse Transcription Master Mix— 1 Tube Product Insert
- Biomark Control Line Fluid 192.24 & 24.192 Product Insert
- Biomark 24.192 GE Dynamic Array Reagent Kit— 10 IFCs Product Insert
- SNP Type Assays and SNP Trace Panel Product Insert
- Delta Gene Assays Product Insert View more
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Training & Services
At Fluidigm, we’re proud to create biotech tools that enable our customers to seek truth in life sciences. We’re dedicated to working with you to empower you. That’s why we’ve developed a range of training programs to help you make the most of your Biomark system.
Fluidigm is dedicated to providing industry-leading technology and tools that enable you to achieve more in life sciences. And to help you stay ahead, we stand behind our work, offering exceptional service plans and options to keep your Biomark instrument in top condition.
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Consumables
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Contact Support
CyTOF & Helios
T: 650 266 6100
Toll Free: 866 358 4354 in the US
Press 5 for CyTOF inquiries when operator is logged offReach out to our global support team here.