The Revolution at Hand
The world’s first automated solution for single-cell genomics research continues to break new ground, now offering more capabilities than ever before. C1 lets you prepare single-cell templates for mRNA sequencing, DNA sequencing, epigenetics or miRNA expression. Reflected in over 100 published studies, the breadth of C1 applications empowers users to survey cell diversity, identify rare cell types and characterize cellular functions, all on the same single-cell biology platform. Investigate tissue heterogeneity with transcript end counting. Use T cell receptor sequencing and other full-length mRNA-seq protocols to recognize isoform- and receptor-specific expression. Examine gene-specific and microRNA expression. Perform epigenetic analysis and identify genomic variants. The C1 system delivers power and flexibility to support you all along your path to discovery.
Learn more about doing bigger, bolder studies with Single-Cell mRNA Seq HT.
PreciseDiscover differences in heterogeneous populations with single-cell accuracy |
SimpleEasily isolate and process individual cells with a streamlined workflow and intuitive interface |
FastMinimize hands-on time with an automated cell-to-data workflow |
FlexibleExplore applications for analyzing DNA, RNA and chromatin, or build your own applications with Script Hub |
Technology
The innovative C1 system carefully isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers or reporter genes. After staining, cells are automatically lysed and template is quickly prepared for qPCR or sequencing analysis—all in as little as four hours.
Workflow
Support
components
IFCs, Assays and Reagents
C1 mRNA Seq HT IFC
Go bigger and bolder—discover novel cell populations or rare cell types with this innovative IFC. Capture and process up to 800 cells per run with on-IFC sample multiplexing and lower library prep costs. Designed to capture cells 10–17 µm in diameter.
C1 DNA Seq IFC
Understand the clonal context of somatic mutations to reveal the true heterogeneity within your sample using the C1 DNA Seq IFC. One integrated workflow lets you isolate and process 96 cells to explore cell lineage at the whole genome, whole exome or targeted loci level. Designed to capture cells 5–25 µm in diameter.
C1 Preamp IFC
The C1 Preamp IFC enables targeted gene expression to verify gene signatures defining a cell population or delve into the miRNA regulating gene expression. Designed to capture cells 5–25 µm in diameter and for use with Biomark systems.
C1 mRNA Seq IFC
Begin exploring cellular heterogeneity with the C1 mRNA Seq IFC. Designed for absolute quantification or full transcript sequencing, our 96-cell IFC gives you access to the whole transcriptome and captures cells 5–25 µm in diameter.
C1 Open App IFC
Create new single-cell applications on the Open App IFC. This innovative IFC is all about flexibility and is designed to capture cells 5–25 µm in diameter, this innovative IFC can be used with the extensive library of C1 applications available through Script Hub™.
Related Systems
Biomark HD
Analyze gene expression with real-time PCR, industry-leading flexibility and consistently high-quality results on the Biomark™ HD system.Polaris
Seamlessly integrate cell biology and molecular analysis at the single-cell level. Within one workflow Polaris™ processes single cells for mRNA sequencing applications.